Surface-enhanced Raman scattering based nonfluorescent probe for multiplex DNA detection

Lan Sun, Chenxu Yu, Joseph Irudayaraj

Research output: Contribution to journalArticlepeer-review


To provide rapid and accurate detection of DNA markers in a straightforward, inexpensive, and multiplex format, an alternative surface-enhanced Raman scattering based probe was designed and fabricated to covalently attach both DNA probing sequence and nonfluorescent Raman tags to the surface of gold nanoparticles (DNA-AuP-RTag). The intensity of Raman signal of the probes could be controlled through the surface coverage of the nonfluorescent Raman tags (RTags). Detection sensitivity of these probes could be optimized by fine-tuning the amount of DNA molecules and RTags on the probes. Long-term stability of the DNA-AuP-RTag probes was found to be good (over 3 months). Excellent multiplexing capability of the DNA-AuP-RTag scheme was demonstrated by simultaneous identification of up to eight probes in a mixture. Detection of hybridization of single-stranded DNA to its complementary targets was successfully accomplished with a long-term goal to use nonfluorescent RTags in a Raman-based DNA microarray platform.

Original languageEnglish (US)
Pages (from-to)3981-3988
Number of pages8
JournalAnalytical Chemistry
Issue number11
StatePublished - Jun 1 2007
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry


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