Surface-enhanced Raman imaging of intracellular bioreduction of chromate in Shewanella oneidensis

Sandeep P. Ravindranath, Kristene L. Henne, Dorothea K. Thompson, Joseph Irudayaraj

Research output: Contribution to journalArticle

Abstract

This proposed research aims to use novel nanoparticle sensors and spectroscopic tools constituting surface-enhanced Raman spectroscopy (SERS) and Fluorescence Lifetime imaging (FLIM) to study intracellular chemical activities within single bioremediating microorganism. The grand challenge is to develop a mechanistic understanding of chromate reduction and localization by the remediating bacterium Shewanella oneidensis MR-1 by chemical and lifetime imaging. MR-1 has attracted wide interest from the research community because of its potential in reducing multiple chemical and metallic electron acceptors. While several biomolecular approaches to decode microbial reduction mechanisms exist, there is a considerable gap in the availability of sensor platforms to advance research from population-based studies to the single cell level. This study is one of the first attempts to incorporate SERS imaging to address this gap. First, we demonstrate that chromate-decorated nanoparticles can be taken up by cells using TEM and Fluorescence Lifetime imaging to confirm the internalization of gold nanoprobes. Second, we demonstrate the utility of a Raman chemical imaging platform to monitor chromate reduction and localization within single cells. Distinctive differences in Raman signatures of Cr(VI) and Cr(III) enabled their spatial identification within single cells from the Raman images. A comprehensive evaluation of toxicity and cellular interference experiments conducted revealed the inert nature of these probes and that they are non-toxic. Our results strongly suggest the existence of internal reductive machinery and that reduction occurs at specific sites within cells instead of at disperse reductive sites throughout the cell as previously reported. While chromate-decorated gold nanosensors used in this study provide an improved means for the tracking of specific chromate interactions within the cell and on the cell surface, we expect our single cell imaging tools to be extended to monitor the interaction of other toxic metal species.

Original languageEnglish (US)
Article numbere16634
JournalPloS one
Volume6
Issue number2
DOIs
StatePublished - Mar 7 2011
Externally publishedYes

Fingerprint

Shewanella oneidensis
Shewanella
Chromates
image analysis
Imaging techniques
cells
Gold
Raman spectroscopy
Raman Spectrum Analysis
Optical Imaging
Fluorescence
nanoparticles
Nanoparticles
Nanoprobes
Nanosensors
gold
Poisons
Sensors
Research
Microorganisms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Surface-enhanced Raman imaging of intracellular bioreduction of chromate in Shewanella oneidensis. / Ravindranath, Sandeep P.; Henne, Kristene L.; Thompson, Dorothea K.; Irudayaraj, Joseph.

In: PloS one, Vol. 6, No. 2, e16634, 07.03.2011.

Research output: Contribution to journalArticle

Ravindranath, Sandeep P. ; Henne, Kristene L. ; Thompson, Dorothea K. ; Irudayaraj, Joseph. / Surface-enhanced Raman imaging of intracellular bioreduction of chromate in Shewanella oneidensis. In: PloS one. 2011 ; Vol. 6, No. 2.
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