Suppressive Subtractive Hybridization Reveals Extensive Horizontal Transfer in the Rumen Metagenome

Elizabeth A. Galbraith, Dionysios A. Antonopoulos, Karen E. Nelson, Bryan A. White

Research output: Chapter in Book/Report/Conference proceedingChapter

Original languageEnglish (US)
Title of host publicationHandbook of Molecular Microbial Ecology I
Subtitle of host publicationMetagenomics and Complementary Approaches
PublisherJohn Wiley & Sons, Ltd.
Number of pages9
ISBN (Print)9780470644799
StatePublished - May 3 2011
Externally publishedYes


  • Dot blot hybridization, Southern dot blot procedure - cloned SSH products, hybridized with tester metagenomic DNA acting as complex probe
  • Quantitative dot blots of rRNA from rumen samples - verifying Archaeal sequences
  • Suppressive subtractive hybridization - extensive horizontal transfer in rumen metagenome

ASJC Scopus subject areas

  • General Immunology and Microbiology

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