TY - JOUR
T1 - Superoxide Dismutases
AU - Beyer, Wayne
AU - Imlay, James
AU - Fridovich, Irwin
PY - 1991/1/1
Y1 - 1991/1/1
N2 - The availability of a spectrophotometric assay for superoxide dismutase (SOD) activity and of an activity stain applicable to polyacrylamide gels facilitated the isolation of SODS from a variety of sources. A manganese-containing SOD (Mn-SOD) was rather quickly isolated from Escherichia coli (13) and from chicken liver mitochondria and an iron-containing SOD (Fe-SOD) was isolated from E. coli. These SODS are widely distributed, abundant and stable, and thus are well-suited for the studies of structure, function, and evolution. In some cases, the distribution of SODS provides a clear indication of evolutionary history, while in other cases remain enigmatic. Mitochondria contain a Mn-SOD, while the cytosols of eukaryotic cells contain a Cu,Zn-SOD. The mitochondria1 Mn-SOD is strikingly homologous to the Mn-SODs/Fe-SODs found in prokaryotes, but bears no resemblance to the cytosolic Cu,Zn-SOD, which supports the idea of an endosymbiotic origin for this organelle.
AB - The availability of a spectrophotometric assay for superoxide dismutase (SOD) activity and of an activity stain applicable to polyacrylamide gels facilitated the isolation of SODS from a variety of sources. A manganese-containing SOD (Mn-SOD) was rather quickly isolated from Escherichia coli (13) and from chicken liver mitochondria and an iron-containing SOD (Fe-SOD) was isolated from E. coli. These SODS are widely distributed, abundant and stable, and thus are well-suited for the studies of structure, function, and evolution. In some cases, the distribution of SODS provides a clear indication of evolutionary history, while in other cases remain enigmatic. Mitochondria contain a Mn-SOD, while the cytosols of eukaryotic cells contain a Cu,Zn-SOD. The mitochondria1 Mn-SOD is strikingly homologous to the Mn-SODs/Fe-SODs found in prokaryotes, but bears no resemblance to the cytosolic Cu,Zn-SOD, which supports the idea of an endosymbiotic origin for this organelle.
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U2 - 10.1016/S0079-6603(08)60843-0
DO - 10.1016/S0079-6603(08)60843-0
M3 - Article
C2 - 1851570
AN - SCOPUS:0025984212
SN - 0079-6603
VL - 40
SP - 221
EP - 253
JO - Progress in nucleic acid research and molecular biology
JF - Progress in nucleic acid research and molecular biology
IS - C
ER -