Super Temporal-Resolved Microscopy (STReM)

Wenxiao Wang; Hao Shen; Bo Shuang; Benjamin S. Hoener; Lawrence J. Tauzin; Nicholas A. Moringo; Kevin F. Kelly; Christy F. Landes

doi:10.1021/acs.jpclett.6b02098

Super Temporal-Resolved Microscopy (STReM)

Wenxiao Wang, Hao Shen, Bo Shuang, Benjamin S. Hoener, Lawrence J. Tauzin, Nicholas A. Moringo, Kevin F. Kelly, Christy F. Landes

Research output: Contribution to journal › Article › peer-review

Abstract

Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquisition and then recovering the temporal information by fitting the point spread function (PSF) orientations. The feasibility of this technique is verified with both simulated and experimental 2D adsorption/desorption and 2D emitter transport. When STReM is applied to measure protein adsorption at a glass surface, previously unseen dynamics are revealed.

Original language	English (US)
Pages (from-to)	4524-4529
Number of pages	6
Journal	Journal of Physical Chemistry Letters
Volume	7
Issue number	22
DOIs	https://doi.org/10.1021/acs.jpclett.6b02098
State	Published - Nov 17 2016
Externally published	Yes

ASJC Scopus subject areas

General Materials Science
Physical and Theoretical Chemistry

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10.1021/acs.jpclett.6b02098

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title = "Super Temporal-Resolved Microscopy (STReM)",

abstract = "Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquisition and then recovering the temporal information by fitting the point spread function (PSF) orientations. The feasibility of this technique is verified with both simulated and experimental 2D adsorption/desorption and 2D emitter transport. When STReM is applied to measure protein adsorption at a glass surface, previously unseen dynamics are revealed.",

author = "Wenxiao Wang and Hao Shen and Bo Shuang and Hoener, {Benjamin S.} and Tauzin, {Lawrence J.} and Moringo, {Nicholas A.} and Kelly, {Kevin F.} and Landes, {Christy F.}",

note = "Funding Information: C.F.L. thanks the Welch Foundation (C-1787) and National Science Foundation (CHE-1151647). Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",

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doi = "10.1021/acs.jpclett.6b02098",

language = "English (US)",

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AU - Wang, Wenxiao

AU - Shen, Hao

AU - Shuang, Bo

AU - Hoener, Benjamin S.

AU - Tauzin, Lawrence J.

AU - Moringo, Nicholas A.

AU - Kelly, Kevin F.

AU - Landes, Christy F.

PY - 2016/11/17

Y1 - 2016/11/17

N2 - Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquisition and then recovering the temporal information by fitting the point spread function (PSF) orientations. The feasibility of this technique is verified with both simulated and experimental 2D adsorption/desorption and 2D emitter transport. When STReM is applied to measure protein adsorption at a glass surface, previously unseen dynamics are revealed.

AB - Super-resolution microscopy typically achieves high spatial resolution, but the temporal resolution remains low. We report super temporal-resolved microscopy (STReM) to improve the temporal resolution of 2D super-resolution microscopy by a factor of 20 compared to that of the traditional camera-limited frame rate. This is achieved by rotating a phase mask in the Fourier plane during data acquisition and then recovering the temporal information by fitting the point spread function (PSF) orientations. The feasibility of this technique is verified with both simulated and experimental 2D adsorption/desorption and 2D emitter transport. When STReM is applied to measure protein adsorption at a glass surface, previously unseen dynamics are revealed.

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Super Temporal-Resolved Microscopy (STReM)

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