Super-Accuracy and Super-Resolution. Getting Around the Diffraction Limit.

Erdal Toprak, Comert Kural, Paul R. Selvin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In many research areas such as biology, biochemistry, and biophysics, measuring distances or identifying and counting objects can be of great importance. To do this, researchers often need complicated and expensive tools in order to have accurate measurements. In addition, these measurements are often done under nonphysiological settings. X-ray diffraction, for example, gets Angstrom-level structures, but it requires crystallizing a biological specimen. Electron microscopy (EM) has about 10 Å resolution, but often requires frozen (liquid nitrogen) samples. Optical microscopy, while coming closest to physiologically relevant conditions, has been limited by the minimum distances to be measured, typically about the diffraction limit, or ~. 200. nm. However, most biological molecules are <. 5-10. nm in diameter, and getting molecular details requires imaging at this scale. In this chapter, we will describe some of the experimental approaches, from our lab and others, that push the limits of localization accuracy and optical resolution in fluorescence microscopy.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages1-26
Number of pages26
EditionC
DOIs
StatePublished - Jan 1 2010

Publication series

NameMethods in Enzymology
NumberC
Volume475
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Toprak, E., Kural, C., & Selvin, P. R. (2010). Super-Accuracy and Super-Resolution. Getting Around the Diffraction Limit. In Methods in Enzymology (C ed., pp. 1-26). (Methods in Enzymology; Vol. 475, No. C). Academic Press Inc.. https://doi.org/10.1016/S0076-6879(10)75001-1