31P NMR spectroscopy of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major. Evidence for high levels of condensed inorganic phosphates

Benjamin Moreno, Julio A. Urbina, Eric Oldfield, Brian N. Bailey, Claudia O. Rodrigues, Roberto Docampo

Research output: Contribution to journalArticle

Abstract

High resolution 31P nuclear magnetic resonance spectra at 303.6 MHz (corresponding to a 1H resonance frequency of 750 MHz) have been obtained of perchloric acid extracts of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major, the causative agents of African sleeping sickness, Chagas' disease, and leishmaniasis. Essentially complete assignments have been made based on chemical shifts and by direct addition of authentic reference compounds. The results indicate the presence of high levels of short chain condensed polyphosphates: di-, tri-, tetra-, and pentapolyphosphate. 31P NMR spectra of purified T. brucei, T. cruzi, and L. major acidocalcisomes, calcium and phosphorus storage organelles, indicate that polyphosphates are abundant in these organelles and have an average chain length of 3.11-3.39 phosphates. In the context of the recent discovery of several pyrophosphate-utilizing enzymes in trypanosomatids, the presence of these inorganic polyphosphates implies a critical role for these molecules in these parasites and a potential new route to chemotherapy.

Original languageEnglish (US)
Pages (from-to)28356-28362
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number37
DOIs
StatePublished - Sep 15 2000

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Leishmania major
Polyphosphates
Trypanosoma brucei brucei
Trypanosoma cruzi
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Phosphates
Organelles
Nuclear magnetic resonance
African Trypanosomiasis
Leishmaniasis
Chemotherapy
Chagas Disease
Chemical shift
Chain length
Phosphorus
Parasites
Calcium
Drug Therapy
Molecules

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

31P NMR spectroscopy of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major. Evidence for high levels of condensed inorganic phosphates. / Moreno, Benjamin; Urbina, Julio A.; Oldfield, Eric; Bailey, Brian N.; Rodrigues, Claudia O.; Docampo, Roberto.

In: Journal of Biological Chemistry, Vol. 275, No. 37, 15.09.2000, p. 28356-28362.

Research output: Contribution to journalArticle

Moreno, Benjamin ; Urbina, Julio A. ; Oldfield, Eric ; Bailey, Brian N. ; Rodrigues, Claudia O. ; Docampo, Roberto. / 31P NMR spectroscopy of Trypanosoma brucei, Trypanosoma cruzi, and Leishmania major. Evidence for high levels of condensed inorganic phosphates. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 37. pp. 28356-28362.
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