31 P NMR of apicomplexans and the effects of risedronate on Cryptosporidium parvum growth

Benjamin Moreno, Brian N. Bailey, Shuhong Luo, Michael B. Martin, Mark S Kuhlenschmidt, Silvia N.J. Moreno, Roberto Docampo, Eric Oldfield

Research output: Contribution to journalArticle

Abstract

High-resolution 303.6 MHz 31 P NMR spectra have been obtained of perchloric acid extracts of Plasmodium berghei trophozoites, Toxoplasma gondii tachyzoites, and Cryptosporidium parvum oocysts. Essentially complete resonance assignments have been made based on chemical shifts and by coaddition of authentic reference compounds. Signals corresponding to inorganic pyrophosphate were detected in all three species. In T. gondii and C. parvum, additional resonances were observed corresponding to linear triphosphate as well as longer chain polyphosphates. Spectra of P. berghei and T. gondii also indicated the presence of phosphomonoesters and nucleotide phosphates. We also report that the pyrophosphate analog drug, risedronate (used in bone resorption therapy), inhibits the growth of C. parvum in a mouse xenograft model. When taken together, our results indicate that all the major disease-causing apicomplexan parasites contain extensive stores of condensed phosphates and that as with Plasmodium falciparum and T. gondii, the pyrophosphate analog drug risedronate is an inhibitor of C. parvum cell growth.

Original languageEnglish (US)
Pages (from-to)632-637
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume284
Issue number3
DOIs
StatePublished - Jan 1 2001

Fingerprint

Cryptosporidium parvum
Toxoplasma
Nuclear magnetic resonance
Plasmodium berghei
Growth
Phosphates
Polyphosphates
Chemical shift
Cell growth
Heterografts
Pharmaceutical Preparations
Trophozoites
Oocysts
Bone
Plasmodium falciparum
Bone Resorption
Nucleotides
Parasites
Risedronate Sodium
diphosphoric acid

Keywords

  • Apicomplexan
  • Bisphosphonate
  • Cryptosporidiosis
  • Malaria
  • NMR
  • Toxoplasmosis
  • Xenograft

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

31 P NMR of apicomplexans and the effects of risedronate on Cryptosporidium parvum growth . / Moreno, Benjamin; Bailey, Brian N.; Luo, Shuhong; Martin, Michael B.; Kuhlenschmidt, Mark S; Moreno, Silvia N.J.; Docampo, Roberto; Oldfield, Eric.

In: Biochemical and Biophysical Research Communications, Vol. 284, No. 3, 01.01.2001, p. 632-637.

Research output: Contribution to journalArticle

Moreno, Benjamin ; Bailey, Brian N. ; Luo, Shuhong ; Martin, Michael B. ; Kuhlenschmidt, Mark S ; Moreno, Silvia N.J. ; Docampo, Roberto ; Oldfield, Eric. / 31 P NMR of apicomplexans and the effects of risedronate on Cryptosporidium parvum growth In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 284, No. 3. pp. 632-637.
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