1H, 13C and 15N chemical shift referencing in biomolecular NMR

David S. Wishart, Colin G. Bigam, Jian Yao, Frits Abildgaard, H. Jane Dyson, Eric Oldfield, John L. Markley, Brian D. Sykes

Research output: Contribution to journalArticlepeer-review


A considerable degree of variability exists in the way that 1H, 13C and 15N chemical shifts are reported and referenced for biomolecules. In this article we explore some of the reasons for this situation and propose guidelines for future chemical shift referencing and for conversion from many common 1H, 13C and 15N chemical shift standards, now used in biomolecular NMR, to those proposed here.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalJournal of Biomolecular NMR
Issue number2
StatePublished - Sep 1995


  • Chemical shift
  • Reference compound
  • Standard

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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