1H NMR Study of the Solution Molecular and Electronic Structure of Escherichia coli Ferricytochrome b562: Evidence for Spin Equilibrium for Intact His/Met Ligation

Jia zhen Wu, Gerd N. La Mar, Liping P. Yu, Kang Bong Lee, F. Ann Walker, Mark L. Chiu, Stephen G. Sligar

Research output: Contribution to journalArticle

Abstract

The solution 500-MHz 1H NMR spectral parameters for ferricytochrome b562, a soluble 12-kDa electron carrier from Escherichia coli with axial His/Met coordination, are shown to be strongly influenced by protein concentration and ionic strength at low pH and 25 °C in a manner consistent with significant aggregation at low ionic strength. At high ionic strength a well-resolved 1H NMR spectrum reveals over 40 hyperfïne-shifted resonances which arise from two isomeric species in the ratio 2:1. 2D COSY and NOESY maps at 25 °C for the hyperfine-shifted resonances allow the assignment of a number of axial His resonances and all heme peripheral substituent peaks. The resulting asymmetric heme contact shift patterns, together with the halving of the number of lines when reconstituting with 2-fold symmetric hemin, demonstrate the molecular basis of the solution heterogeneity to be heme orientational disorder. The strongly upfield-shifted axial Met-7 resonances, characteristic of low-spin ferricytochromes c with His/Met ligation, appear upfield only at very low temperatures. At elevated temperatures, all resonances, in particular those of the axial Met, move strongly downfield. Detailed analysis of the deviation from Curie behavior for different functional groups demonstrates the presence of a low spin ⇄ high spin equilibrium with an intact His–Fe–Met coordination. The weaker axial field in ferricytochrome b562, relative to the purely low-spin ferricytochromes c, is attributed to a perturbed iron-Met bond. The contact shifts for a coordinated Met in the high-spin state are estimated. A link between equatorial hemin and axial ligand interactions is indicated by a differential population of the high-spin form for the two hemin orientations.

Original languageEnglish (US)
Pages (from-to)2156-2165
Number of pages10
JournalBiochemistry
Volume30
Issue number8
DOIs
StatePublished - Feb 1 1991

ASJC Scopus subject areas

  • Biochemistry

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