TY - JOUR
T1 - 1H-Detected REDOR with Fast Magic-Angle Spinning of a Deuterated Protein
AU - Ghosh, Manali
AU - Rienstra, Chad M.
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/9/14
Y1 - 2017/9/14
N2 - Rotational echo double resonance (REDOR) is a highly successful method for heteronuclear distance determination in biological solid-state NMR, and 1H detection methods have emerged in recent years as a powerful approach to improving sensitivity and resolution for small sample quantities by utilizing fast magic-angle spinning (>30 kHz) and deuteration strategies. In theory, involving 1H as one of the spins for measuring REDOR effects can greatly increase the distance measurement range, but few experiments of this type have been reported. Here we introduce a pulse sequence that combines frequency-selective REDOR (FSR) with 1H detection. We demonstrate this method with applications to samples of uniformly 13C,15N,2H-labeled alanine and uniformly 13C,2H,15N-labeled GB1 protein, back-exchanged with 30% H2O and 70% D2O, employing a variety of frequency-selective 13C pulses to highlight unique spectral features. The resulting, robust REDOR effects provide (1) tools for resonance assignment, (2) restraints of secondary structure, (3) probes of tertiary structure, and (4) approaches to determine the preferred orientation of aromatic rings in the protein core.
AB - Rotational echo double resonance (REDOR) is a highly successful method for heteronuclear distance determination in biological solid-state NMR, and 1H detection methods have emerged in recent years as a powerful approach to improving sensitivity and resolution for small sample quantities by utilizing fast magic-angle spinning (>30 kHz) and deuteration strategies. In theory, involving 1H as one of the spins for measuring REDOR effects can greatly increase the distance measurement range, but few experiments of this type have been reported. Here we introduce a pulse sequence that combines frequency-selective REDOR (FSR) with 1H detection. We demonstrate this method with applications to samples of uniformly 13C,15N,2H-labeled alanine and uniformly 13C,2H,15N-labeled GB1 protein, back-exchanged with 30% H2O and 70% D2O, employing a variety of frequency-selective 13C pulses to highlight unique spectral features. The resulting, robust REDOR effects provide (1) tools for resonance assignment, (2) restraints of secondary structure, (3) probes of tertiary structure, and (4) approaches to determine the preferred orientation of aromatic rings in the protein core.
UR - http://www.scopus.com/inward/record.url?scp=85029495343&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029495343&partnerID=8YFLogxK
U2 - 10.1021/acs.jpcb.7b07313
DO - 10.1021/acs.jpcb.7b07313
M3 - Article
C2 - 28816462
AN - SCOPUS:85029495343
SN - 1520-6106
VL - 121
SP - 8503
EP - 8511
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
IS - 36
ER -