In this Communication, we demonstrate the use of deuteration together with back substitution of exchangeable protons as a means of attenuating the strong 1 H- 1 H couplings that broaden 1 H magic angle spinning (MAS) spectra of solids. The approach facilitates 15 N- 1 H correlation experiments as well as experiments for the measurement of 1 H- 1 H distances. The distance measurement relies on the excellent resolution in the 1 H MAS spectrum and homonuclear double quantum recoupling techniques. The 1 H- 1 H dipolar recoupling can be analyzed in an analytical fashion by fitting the data to a 2- or 3-spin system. The experiments are performed on a sample of the dipeptide N-Ac-Val-Leu-OH, which was synthesized from uniformly [ 2 H, 15 N] labeled materials and back-exchanged in H 2 O.
ASJC Scopus subject areas
- Nuclear and High Energy Physics
- Condensed Matter Physics