SunnyTALEN: A second-generation TALEN system for human genome editing

Ning Sun, Zehua Bao, Xiong Xiong, Huimin Zhao

Research output: Contribution to journalArticlepeer-review

Abstract

Transcription activator-like effector nucleases (TALENs) have rapidly emerged as a powerful genome editing tool. The site-specific DNA double-strand breaks generated by TALENs in the human chromosome can induce homologous recombination or non-homologous end joining, resulting in desired genetic modifications. In this study, we report the development of a TALEN variant, SunnyTALEN, with >2.5-fold improved genome editing efficacy in human cells. The corresponding scaffold increases the rate of genetic modification at all the 13 tested loci of human genome and is compatible with heterodimer TALEN architectures. This enhanced and high-efficiency TALEN variant represents a novel second-generation TALEN system and has great potential for biological and therapeutic applications. Biotechnol. Bioeng. 2014;111: 683-691.

Original languageEnglish (US)
Pages (from-to)683-691
Number of pages9
JournalBiotechnology and bioengineering
Volume111
Issue number4
DOIs
StatePublished - Apr 2014

Keywords

  • Directed evolution
  • Double-strand break
  • Gene therapy
  • Genome editing
  • Synthetic biology

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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