@article{c327de6e2760494488cf915c77d4d376,
title = "Sucrose density-gradient analysis of Euglena gracilis RNA isolated with Macaloid",
author = "Schuit, {K. E.} and Buetow, {D. E.}",
note = "Funding Information: 17.5 ~/o of the total RNA. Preliminary labeling experiments with 3*Pl show that the molecules sedimenting in this region are more rapidly labeled than the rRNA and appear, therefore, to represent a distinct class of RNA. The function and nature of the I3-S RNA is unknown and is presently under study. In the present experiments when the RNA from streptomycin-bleached Euglena was isolated by the method of BRAWERMAN AND EISENSTADT3 , in the absence of Macaloid, the high molecular weight RNA sedimented in a single peak. When Macaloid was added to the extraction mixture, the peak of high molecular weight RNA previously sedimenting at 19 S (refs. 3-5) was resolved into two classes of RNA with sedimentation coefficients of 17 and 22 (Fig. I). We classify these high molecular weight t~NA's as rRNA for two reasons. First, their sedimentation coefficients (17 S and 22 S) fall within the range of commonly reported values for the rRNA's of other organisms. Secondly, the isolated heavy (22 S) and light (17 S) molecules are present in approximately a 2:1 proportion. The present results suggest that the single I9-S rRNA a-5 from Euglena results from ribonuclease degradation during RNA isolation. By using Macaloid to remove the ribonuclease activity of the cell homogenates we have been able to isolate the high molecular weight RNA in an intact form. This work was supported by grant HD o3163 from the National Institute of Child Health and Human Development. The authors are grateful to Drs. W. VINCENT and R. TABOR of the University of Pittsburgh for helpful suggestions and to Mr. CLYDE MARTIN of the National Lead Co., Houston, Texas, for the Macaloid.",
year = "1968",
month = oct,
day = "29",
doi = "10.1016/0005-2787(68)90378-X",
language = "English (US)",
volume = "166",
pages = "702--704",
journal = "BBA Section Nucleic Acids And Protein Synthesis",
issn = "0005-2787",
publisher = "Elsevier B.V.",
number = "3",
}