Subunit stoichiometry of a heteromultimeric G protein-coupled inward- rectifier K+ channel

Scott K. Silverman, Henry A. Lester, Dennis A. Dougherty

Research output: Contribution to journalArticle

Abstract

We investigated the stoichiometry of the heteromultimeric G protein- coupled inward-rectifier K+ channel (GIRK) formed from GIRK1 and GIRK4 subunits. Multimeric GIRK constructs with several concatenated channel subunits were expressed in Xenopus oocytes. Coexpression of various trimeric constructs with different monomers clearly showed that the functional channel has stoichiometry (GIRK1)2(GIRK4)2. Efforts to establish a preferred arrangement of subunits around the channel pore suggest that more than one arrangement may be viable.

Original languageEnglish (US)
Pages (from-to)30524-30528
Number of pages5
JournalJournal of Biological Chemistry
Volume271
Issue number48
DOIs
StatePublished - 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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