Substrains of 129 mice are resistant to Yersinia pestis KIM5: Implications for interleukin-10-deficient mice

Joshua K. Turner, John L. Xu, Richard I. Tapping

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Interleukin-10 (IL-10)-deficient mice are resistant to several pathogens, including Yersinia pestis. Surprisingly, we observed that heterozygous IL-10+/- mice also survive high-dose intravenous infection with Y. pestis KIM5 (Pgm-). Analysis of commercial IL-10-/- mice revealed that at least 30 cM of genomic DNA from the original 129 strain remains, including a functional Slc11a1 (Nramp1) gene. Interestingly, two substrains of 129 mice were resistant to high-dose Y. pestis KIM5. Resistance does not appear to be recessive, as F1 mice (C57BL/6J x 129) also survived a high-dose challenge. A QTL-based genetic scan of chromosome 1 with 35 infected F1 backcrossed mice revealed that resistance to KIM5 maps to a region near IL-10. Two novel IL-10+/+ mouse strains which each possess most of the original 30-cM stretch of 129 DNA maintained resistance to high-dose infection with Y. pestis KIM5 even in a heterozygous state. Conversely, a novel IL-10-/- mouse strain in which most of the 129 DNA has been crossed out exhibited intermediate resistance to KIM5, while the corresponding IL-10+/- strain was completely susceptible. Taken together, these results demonstrate that 129-derived genomic DNA near IL-10 confers resistance to Yersinia pestis KIM5 and contributes to the observed resistance of IL-10-/- mice.

    Original languageEnglish (US)
    Pages (from-to)367-373
    Number of pages7
    JournalInfection and immunity
    Volume77
    Issue number1
    DOIs
    StatePublished - Jan 2009

    ASJC Scopus subject areas

    • Parasitology
    • Microbiology
    • Immunology
    • Infectious Diseases

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