Submicrosecond real-time fluorescence sampling: Application to protein folding

John Ervin, Jobiah Sabelko, Martin Gruebele

Research output: Contribution to journalReview article

Abstract

Time-resolved fluorescence detection has become a central tool in the study of protein folding. This article briefly reviews modern fluorescence techniques and then focuses on recent improvements made possible by array photomultipliers, computer-controlled data gating, and long-memory multi-channel digitizers. It is now possible to detect fluorescence wavelength profiles and/or fluorescence decay transients very cost effectively with sub-microsecond kinetic time resolution out to long times. Folding kinetics can be analyzed by singular value decomposition (SVD) or χ-analysis. The latter provides an objective method for detecting nonexponential kinetics in two-state systems. (C) 2000 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)1-15
Number of pages15
JournalJournal of Photochemistry and Photobiology B: Biology
Volume54
Issue number1
DOIs
StatePublished - Jan 30 2000

Keywords

  • Apomyoglobin
  • Nonexponential kinetics
  • Phosphoglycerate kinase
  • Tryptophan
  • Ubiquitin

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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