Subchronic and low dose of tributyltin exposure leads to reduced ovarian reserve, reduced uterine gland number, and other reproductive irregularities in female mice

Isabela V. Sarmento, Eduardo Merlo, Silvana S. Meyrelles, Elisardo C. Vasquez, Genoa R. Warner, Andressa Gonsioroski, Kathy De La Torre, Daryl D. Meling, Jodi A. Flaws, Jones B. Graceli

Research output: Contribution to journalArticlepeer-review

Abstract

Tributyltin (TBT) chloride is an endocrine disrupting chemical associated with reproductive complications. Studies have shown that TBT targets the reproductive tract, impairing ovarian folliculogenesis, and uterine morphophysiology. In this investigation, we assessed whether subchronic and low dose of TBT exposure results in abnormal ovarian follicular reserve and other irregularities in female mice. TBT was administered to female mice (500 ng/kg/day for 12 days via gavage), and reproductive tract morphophysiology was assessed.We further assessed reproductive tract inflammation and oxidative stress. Improper functioning of the reproductive tract in TBT mice was observed. Specifically, irregular estrous cyclicity and abnormal ovarian morphology coupled with reduction in primordial and primary follicle numbers was observed, suggesting ovarian reserve depletion. In addition, improper follicular development and a reduction in antral follicles, corpora lutea, and total healthy ovarian follicles together with an increase in cystic follicles were apparent. Evidence of uterine atrophy, reduction in endometrial gland number, and inflammation and oxidative stress were seen in TBT mice. Further, strong negative correlations were observed between testosterone levels and primordial, primary, and total healthy ovarian follicles. Thus, these data suggest that the subchronic and low dose of TBT exposure impaired ovarian follicular reserve, uterine gland number, and other reproductive features in female mice.

Original languageEnglish (US)
Pages (from-to)74-85
Number of pages12
JournalToxicological Sciences
Volume176
Issue number1
DOIs
StatePublished - Jul 1 2020

Keywords

  • Endocrine disruptor chemical
  • Hyperandrogenism
  • Ovarian reserve
  • Tributyltin
  • Uterine glands

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Subchronic and low dose of tributyltin exposure leads to reduced ovarian reserve, reduced uterine gland number, and other reproductive irregularities in female mice'. Together they form a unique fingerprint.

Cite this