TY - JOUR
T1 - Subcellular localization of the porcine deltacoronavirus nucleocapsid protein
AU - Ding, Zhen
AU - Luo, Suxian
AU - Gong, Wang
AU - Wang, Leyi
AU - Ding, Nengshui
AU - Chen, Jun
AU - Chen, Jiajia
AU - Wang, Ting
AU - Ye, Yu
AU - Song, Deping
AU - Kong, Lingbao
AU - Zhang, Jinghua
AU - Tang, Yuxing
N1 - Funding Information:
This work was supported by grants from The National Natural Science Foundation of China (Grant No. 31860704), Jiangxi Province Project (Grant Nos. GJJ170261, 20192BAB214023) and Program for Jiangxi Agriculture Research System (Grant No. JXARS-01). We thank Katie Oakley, PhD, from Liwen Bianji, Edanz Editing China ( www.liwenbianji.cn/ac ), for editing the English text of a draft of this manuscript.
Publisher Copyright:
© 2020, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2020/12
Y1 - 2020/12
N2 - Porcine deltacoronavirus (PDCoV) has been recently identified as an emerging enteropathogenic coronavirus that mainly infects newborn piglets and causes enteritis, diarrhea and high mortality. Although coronavirus N proteins have multifarious activities, the subcellular localization of the PDCoV N protein is still unknown. Here, we produced mouse monoclonal antibodies against the PDCoV N protein. Experiments using anti-haemagglutinin antibodies and these monoclonal antibodies revealed that the PDCoV N protein is shuttled into the nucleolus in both ectopic PDCoV N-expressing cells and PDCoV-infected cells. The results of deletion mutagenesis experiments demonstrated that the predicted nucleolar localization signal at amino acids 295–318 is critical for nucleolar localization. Cumulatively, our study yielded a monoclonal antibody against the PDCoV N protein and revealed a mechanism by which the PDCoV N protein translocated into the nucleolus. The tolls and findings from this work will facilitate further investigations on the functions of the PDCoV N protein.
AB - Porcine deltacoronavirus (PDCoV) has been recently identified as an emerging enteropathogenic coronavirus that mainly infects newborn piglets and causes enteritis, diarrhea and high mortality. Although coronavirus N proteins have multifarious activities, the subcellular localization of the PDCoV N protein is still unknown. Here, we produced mouse monoclonal antibodies against the PDCoV N protein. Experiments using anti-haemagglutinin antibodies and these monoclonal antibodies revealed that the PDCoV N protein is shuttled into the nucleolus in both ectopic PDCoV N-expressing cells and PDCoV-infected cells. The results of deletion mutagenesis experiments demonstrated that the predicted nucleolar localization signal at amino acids 295–318 is critical for nucleolar localization. Cumulatively, our study yielded a monoclonal antibody against the PDCoV N protein and revealed a mechanism by which the PDCoV N protein translocated into the nucleolus. The tolls and findings from this work will facilitate further investigations on the functions of the PDCoV N protein.
KW - Porcine deltacoronavirus
KW - Nucleolar localization
KW - mAb
KW - Nucleocapsid protein
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U2 - 10.1007/s11262-020-01790-0
DO - 10.1007/s11262-020-01790-0
M3 - Article
C2 - 32944812
SN - 1572-994X
VL - 56
SP - 687
EP - 695
JO - Virus Genes
JF - Virus Genes
IS - 6
ER -