Sub-diffraction-limited imaging by photobleaching imprinting microscopy

Liang Gao, Alejandro Garcia-Uribe, Yan Liu, Chiye Li, Lihong Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We present a generic sub-diffraction-limited imaging method - photobleaching imprinting microscopy (PIM) - for biological fluorescence imaging. A lateral resolution of 110 nm was measured, more than a two-fold improvement over the optical diffraction limit. Unlike other super-resolution imaging techniques, PIM does not require complicated illumination modules or specific fluorescent dyes. PIM is expected to facilitate the conversion of super-resolution imaging into a routine lab tool, making it accessible to a much broader biological research community.

Original languageEnglish (US)
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging VII
ISBN (Print)9780819498632
StatePublished - 2014
EventSingle Molecule Spectroscopy and Superresolution Imaging VII - San Francisco, CA, United States
Duration: Feb 1 2014Feb 2 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherSingle Molecule Spectroscopy and Superresolution Imaging VII
CountryUnited States
CitySan Francisco, CA


  • Fluorescence microscopy
  • Super-resolution microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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