Abstract
We studied θ-mode DNA replication in p15A-based Escherichia coli plasmids by analyzing their replication intermediates using a combination of neutral agarose 2D gel electrophoresis and electron microscopy. Our analysis: (1) confirms the original assignment of various features of the 2D gel pattern; (2) shows that while one replication fork progresses around the plasmid DNA, the other is immobile, as if the replication were unidirectional; and (3) reveals that termination often occurs at a location away from the replication origin, suggesting that the replication of our plasmids is, in fact, bidirectional, the two forks being active at different times.
Original language | English (US) |
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Pages (from-to) | 1-7 |
Number of pages | 7 |
Journal | Journal of Molecular Biology |
Volume | 268 |
Issue number | 1 |
DOIs | |
State | Published - Apr 25 1997 |
Externally published | Yes |
Keywords
- 2D agarose gel-electrophoresis
- Electron microscopy
- P15A replicon
- Replication intermediates
- Unidirectional replication
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology