Study of microbial community of brewery-treating granular sludge by denaturing gradient gel electrophoresis of 16S rRNA gene

O. C. Chan, W. T. Liu, H. H. Fang

Research output: Contribution to journalArticlepeer-review

Abstract

The microbial community structure of granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating brewery effluent was studied by denaturing gradient gel electrophoresis (DGGE). Twelve major bands were observed in the DGGE fingerprint for the Bacteria domain and four bands for the Archaea domain. Of the bacterial bands observed, six were successfully purified and sequenced. Among them, three were related to the gram-positive low G+C group, one to the Delta subclass of the Proteobacteria, one to the Gamma subclass, and one to the Cytophaga group with no close related sequence. The 16S rRNA sequences of the four archaeal bands were closely associated with Methanosaeta concilii and Methanobacterium formicum.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalWater Science and Technology
Volume43
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • 16S rRNA
  • DGGE
  • Granule
  • Microbial community
  • USAB

ASJC Scopus subject areas

  • Environmental Engineering
  • Water Science and Technology

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