Studies on the genotoxic effects of aminophenazines using two cellular models and three different methods

A. Cebulska-Wasilewska; D. Nowak; W. Niedzwiedz; Elizabeth D. Wagner; Michael J. Plewa

doi:10.1016/S1383-5718(99)00149-7

Studies on the genotoxic effects of aminophenazines using two cellular models and three different methods

A. Cebulska-Wasilewska, D. Nowak, W. Niedzwiedz, Elizabeth D. Wagner, Michael J. Plewa

Research output: Contribution to journal › Article › peer-review

Abstract

This paper presents studies on the genotoxicity of two aminophenazines: 2,3-diaminophenazine (DAP) and 2-amino-3-hydroxyphenazine (AHP). The genotoxic activities of these compounds were evaluated with human lymphocytes using the alkaline single cell gel electrophoresis (SCGE) assay and two cytogenetic assays (chromosome aberrations (CA) and sister chromatid exchange (SCE) analysis). Results show that these chemicals elicited an increase in DNA and chromosomal damage under the studied ranges of concentration. Concentration-response curves were similar and there was a positive correlation between the damage observed at the DNA and chromosomal levels. DAP was more genotoxic than AHP and this agreed with the genotoxic potencies reported in bacterial systems. Copyright (C) 1999 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	57-65
Number of pages	9
Journal	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume	446
Issue number	1
DOIs	https://doi.org/10.1016/S1383-5718(99)00149-7
State	Published - Oct 29 1999
Externally published	Yes

Keywords

Aminophenazines
Human lymphocytes
Single cell gel electrophoresis

ASJC Scopus subject areas

Genetics
Health, Toxicology and Mutagenesis

Online availability

10.1016/S1383-5718(99)00149-7

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title = "Studies on the genotoxic effects of aminophenazines using two cellular models and three different methods",

abstract = "This paper presents studies on the genotoxicity of two aminophenazines: 2,3-diaminophenazine (DAP) and 2-amino-3-hydroxyphenazine (AHP). The genotoxic activities of these compounds were evaluated with human lymphocytes using the alkaline single cell gel electrophoresis (SCGE) assay and two cytogenetic assays (chromosome aberrations (CA) and sister chromatid exchange (SCE) analysis). Results show that these chemicals elicited an increase in DNA and chromosomal damage under the studied ranges of concentration. Concentration-response curves were similar and there was a positive correlation between the damage observed at the DNA and chromosomal levels. DAP was more genotoxic than AHP and this agreed with the genotoxic potencies reported in bacterial systems. Copyright (C) 1999 Elsevier Science B.V.",

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author = "A. Cebulska-Wasilewska and D. Nowak and W. Niedzwiedz and Wagner, {Elizabeth D.} and Plewa, {Michael J.}",

note = "Funding Information: Research was partially supported by contract ERBIC 15CT960300 of the Commission of the European Communities. We would like to thank Prof. K. Chru{\'s}cielska from the Institute of Organic Industry, Warsaw, for supplying aminophenazines. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.",

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AU - Cebulska-Wasilewska, A.

AU - Nowak, D.

AU - Niedzwiedz, W.

AU - Wagner, Elizabeth D.

AU - Plewa, Michael J.

N1 - Funding Information: Research was partially supported by contract ERBIC 15CT960300 of the Commission of the European Communities. We would like to thank Prof. K. Chruścielska from the Institute of Organic Industry, Warsaw, for supplying aminophenazines. Copyright: Copyright 2007 Elsevier B.V., All rights reserved.

PY - 1999/10/29

Y1 - 1999/10/29

N2 - This paper presents studies on the genotoxicity of two aminophenazines: 2,3-diaminophenazine (DAP) and 2-amino-3-hydroxyphenazine (AHP). The genotoxic activities of these compounds were evaluated with human lymphocytes using the alkaline single cell gel electrophoresis (SCGE) assay and two cytogenetic assays (chromosome aberrations (CA) and sister chromatid exchange (SCE) analysis). Results show that these chemicals elicited an increase in DNA and chromosomal damage under the studied ranges of concentration. Concentration-response curves were similar and there was a positive correlation between the damage observed at the DNA and chromosomal levels. DAP was more genotoxic than AHP and this agreed with the genotoxic potencies reported in bacterial systems. Copyright (C) 1999 Elsevier Science B.V.

AB - This paper presents studies on the genotoxicity of two aminophenazines: 2,3-diaminophenazine (DAP) and 2-amino-3-hydroxyphenazine (AHP). The genotoxic activities of these compounds were evaluated with human lymphocytes using the alkaline single cell gel electrophoresis (SCGE) assay and two cytogenetic assays (chromosome aberrations (CA) and sister chromatid exchange (SCE) analysis). Results show that these chemicals elicited an increase in DNA and chromosomal damage under the studied ranges of concentration. Concentration-response curves were similar and there was a positive correlation between the damage observed at the DNA and chromosomal levels. DAP was more genotoxic than AHP and this agreed with the genotoxic potencies reported in bacterial systems. Copyright (C) 1999 Elsevier Science B.V.

KW - Aminophenazines

KW - Human lymphocytes

KW - Single cell gel electrophoresis

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Studies on the genotoxic effects of aminophenazines using two cellular models and three different methods

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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