Abstract
Semiconductor quantum dots were used for the first time to label trichosanthin. The absorption spectrum, fluorescent spectrum and enzymatic activity of quantum dots-labeled trichosanthin were studied in this paper. The absorbane of QDs-TCS varied to a less extent with wavelength in the range of 400-600 nm. The fluorescent spectrum of QDs-TCS underwent a blue-shift in the emission peak, but the intrinsic spectral width was unchanged. No changes were found in the enzymatic activity of QDs-TCS in comparison with trichosanthin. Distribution of QDs-TCS within human choriocarcinoma cells was simultaneously observed with two-photon laser scanning microscopy and confocal laser scanning microscopy. The results indicated that QDs-TCS could enter the cytoplasm across the membrane and aggregated around the nuleolus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 36-37 |
| Number of pages | 2 |
| Journal | Kao Teng Hsueh Hsiao Hua Heush Hsueh Pao/ Chemical Journal of Chinese Universities |
| Volume | 22 |
| Issue number | 1 |
| State | Published - Jan 10 2001 |
| Externally published | Yes |
Keywords
- Enzymatic activevity
- Quantum dots
- Trichosanthin
ASJC Scopus subject areas
- General Chemistry