Studies of the degradation products of nisin, a peptide antibiotic, using capillary electrophoresis with off-line mass spectrometry

Lou Ann Cruz, Rebecca W. Garden, Herbert J. Kaiser, Jonathan V. Sweedler

Research output: Contribution to journalArticlepeer-review

Abstract

The utility of capillary electrophoresis (CE) for assessing the purity and stability of pharmaceutical peptides is investigated. The degradation of nisin, a pentacyclic peptide antibiotic, depends upon sample preparation and storage conditions and is followed by CE. With conventional UV detection, peaks are not identified and unresolved components are not detected. Matrix- assisted laser desorption ionization time-of-flight mass spectrometry (MALDI- TOFMS) of isolated CE fractions provides molecular mass information that aids in identification of the nisin degradants and determination of peak purity. The purity of pure and degraded nisin in the absence of any separation is also determined using electrospray ionization mass spectrometry (ESI-MS) and MALDI-TOFMS.

Original languageEnglish (US)
Pages (from-to)375-385
Number of pages11
JournalJournal of Chromatography A
Volume735
Issue number1-2
DOIs
StatePublished - May 31 1996

Keywords

  • Antibiotics
  • Capillary electrophoresis-mass spectrometry
  • Nisin
  • Peptides

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Studies of the degradation products of nisin, a peptide antibiotic, using capillary electrophoresis with off-line mass spectrometry'. Together they form a unique fingerprint.

Cite this