TY - JOUR
T1 - Structure of the yeast endoplasmic reticulum
T2 - Localization of ER proteins using immunofluorescence and immunoelectron microscopy
AU - Preuss, Daphne
AU - Mulholland, Jon
AU - Kaiser, Chris A.
AU - Orlean, Peter
AU - Albright, Charles
AU - Rose, Mark D.
AU - Robbins, Phillips W.
AU - Botstein, David
PY - 1991/12
Y1 - 1991/12
N2 - The endoplasmic reticulum (ER) and other secretory compartments of Saccharomyces cerevisiae have biochemical functions that closely parallel those described in higher eukaryotic cells, yet the morphology of the yeast organelles is quite distinct. In order to associate ER functions with the corresponding cellular structures, we localized several proteins, each of which is expected to be associated with the ER on the basis of enzymatic activity, biological function, or oligosaccharide content. These marker proteins were visualized by immunofluorescence or immunoelectron microscopy, allowing definition of the S. cerevisiae ER structure, both in intact cells and at the ultrastructural level. Each marker protein was most abundant within the membranes that envelop the nucleus and several were also found in extensions of the ER that frequently juxtapose the plasma membrane. Double‐labeling experiments were entirely consistent with the idea that the marker proteins reside within the same compartment. This analysis has permitted, for the first time, a detailed characterization of the ER morphology as yeast cells proceed through their growth and division cycles.
AB - The endoplasmic reticulum (ER) and other secretory compartments of Saccharomyces cerevisiae have biochemical functions that closely parallel those described in higher eukaryotic cells, yet the morphology of the yeast organelles is quite distinct. In order to associate ER functions with the corresponding cellular structures, we localized several proteins, each of which is expected to be associated with the ER on the basis of enzymatic activity, biological function, or oligosaccharide content. These marker proteins were visualized by immunofluorescence or immunoelectron microscopy, allowing definition of the S. cerevisiae ER structure, both in intact cells and at the ultrastructural level. Each marker protein was most abundant within the membranes that envelop the nucleus and several were also found in extensions of the ER that frequently juxtapose the plasma membrane. Double‐labeling experiments were entirely consistent with the idea that the marker proteins reside within the same compartment. This analysis has permitted, for the first time, a detailed characterization of the ER morphology as yeast cells proceed through their growth and division cycles.
KW - Saccharomyces cerevisae
KW - Yeast
KW - cell cycle
KW - endoplasmic reticulum
KW - ultrastructure
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U2 - 10.1002/yea.320070902
DO - 10.1002/yea.320070902
M3 - Article
C2 - 1803815
AN - SCOPUS:0026335172
VL - 7
SP - 891
EP - 911
JO - Yeast
JF - Yeast
SN - 0749-503X
IS - 9
ER -