Structure of replicating herpes simplex virus DNA

C. V. Jongeneel, S. L. Bachenheimer

Research output: Contribution to journalArticlepeer-review


We have investigated the molecular anatomy of the herpes simplex virus replicative intermediates by cleavage with the restriction endonuclease BglII. We find that in populations of multiply infected cells, pulse-labeled replicating herpes simplex virus DNA contains at least two and probably all four sequence isomers. Also, it contains no detectable termini. In pulse-chase experiments, we show that endless replicative intermediates are the precursors to virion DNA and that maturation is a relatively slow process. The results are discussed in terms of their significance to possible models of herpes simplex virus DNA replication.

Original languageEnglish (US)
Pages (from-to)656-660
Number of pages5
JournalJournal of virology
Issue number2
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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