Structure of a proteasome Pba1-Pba2 complex implications for proteasome assembly, activation, and biological function

Beth M. Stadtmueller; Erik Kish-Trier; Katherine Ferrell; Charisse N. Petersen; Howard Robinson; David G. Myszka; Debra M. Eckert; Tim Formosa; Christopher P. Hill

doi:10.1074/jbc.M112.367003

Structure of a proteasome Pba1-Pba2 complex implications for proteasome assembly, activation, and biological function

Beth M. Stadtmueller, Erik Kish-Trier, Katherine Ferrell, Charisse N. Petersen, Howard Robinson, David G. Myszka, Debra M. Eckert, Tim Formosa, Christopher P. Hill

Research output: Contribution to journal › Article › peer-review

Abstract

Pba1-Pba2 facilitates proteasome α-ring assembly. Results: Pba1-Pba2 binds mature proteasomes using C-terminal motifs and sequesters α-subunitNtermini. It does not activate and is not degraded by isolated 20S proteasomes. Conclusion: Pba1-Pba2 is important for proteasome-dependent maintenance of mitochondrial function. The structure is consistent with multiple roles in proteasome assembly. Significance: Models of proteasome assembly and Pba1-Pba2 proteasome function are advanced.

Original language	English (US)
Pages (from-to)	37371-37382
Number of pages	12
Journal	Journal of Biological Chemistry
Volume	287
Issue number	44
DOIs	https://doi.org/10.1074/jbc.M112.367003
State	Published - Oct 26 2012
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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Structure of a proteasome Pba1-Pba2 complex implications for proteasome assembly, activation, and biological function. / Stadtmueller, Beth M.; Kish-Trier, Erik; Ferrell, Katherine; Petersen, Charisse N.; Robinson, Howard; Myszka, David G.; Eckert, Debra M.; Formosa, Tim; Hill, Christopher P.

In: Journal of Biological Chemistry, Vol. 287, No. 44, 26.10.2012, p. 37371-37382.

Research output: Contribution to journal › Article › peer-review

Stadtmueller, Beth M. ; Kish-Trier, Erik ; Ferrell, Katherine ; Petersen, Charisse N. ; Robinson, Howard ; Myszka, David G. ; Eckert, Debra M. ; Formosa, Tim ; Hill, Christopher P. / Structure of a proteasome Pba1-Pba2 complex implications for proteasome assembly, activation, and biological function. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 44. pp. 37371-37382.

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AU - Robinson, Howard

AU - Myszka, David G.

AU - Eckert, Debra M.

AU - Formosa, Tim

AU - Hill, Christopher P.

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