We have employed small angle x-ray scattering to examine the solution conformation of cytochrome P-450cam in the presence and absence of its substrate, camphor. The radius of gyration (Rg) of the protein is found to be 23.9 +/- 0.2 A in both states. Comparison of the radius of gyration with that expected for a spherical protein of the same molecular weight indicates that P-450 is an elongated molecule and that its shape may best be approximated by a cylinder about 30 A in diameter and 80 A in length.
|Original language||English (US)|
|Number of pages||3|
|Journal||Journal of Biological Chemistry|
|State||Published - Mar 25 1983|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology