Structural sequences are conserved in the genes coding for the α, α′ and β-subunits of the soybean 7S seed storage protein

Mary A. Schuler, Beth F. Ladin, Joseph C. Pollaco, Gregory Freyer, Roger N. Beachy

Research output: Contribution to journalArticle

Abstract

Cloned DNAs encoding four different proteins have been isolated from recombinant cDNA libraries constructed with Glycine max seed mRNAs. Two cloned DNAs code for the α andα′-subunits of the 7s seed storage protein (conglycinin). The other cloned cDNAs code for proteins which are synthesized in vitro as 68, 000d., 60,000 d. or 53,000 d.polypeptides. Hybrid selection experiments indicate that, under low stringency hybridization conditions, all four cDNAs hybridize with mRNAs for the α and α′-subunits and the 68,000d., 60,000d. and 53,000d. in vitro translation products. Within three of the mRNA, there is a conserved sequence of 155 nucleotides which is responsible for this hybridization. The conserved nucleotides in the α and α′-subunit cDNAs and the 68,000d. polypeptide cDNAs span both coding and noncoding sequences. The differences in the coding nucleotides outside the conserved region are extensive. This suggests that selective pressure to maintain the155 conserved nucleotides has been influenced by the structure of the seed mRNA. RNA blot hybridizations demonstrate that mRNA encoding the other major subunit(β)of the 7s seed storage protein also shares sequence homology with the conserved 155 nucleotide sequence of the αand α′-subunit mRNAs, but not with other coding sequences.

Original languageEnglish (US)
Pages (from-to)8245-8261
Number of pages17
JournalNucleic acids research
Volume10
Issue number24
DOIs
StatePublished - Dec 20 1982
Externally publishedYes

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Seed Storage Proteins
Conserved Sequence
Soybeans
Messenger RNA
Nucleotides
Complementary DNA
Genes
Seeds
Peptides
DNA
Sequence Homology
Gene Library
Proteins
RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Structural sequences are conserved in the genes coding for the α, α′ and β-subunits of the soybean 7S seed storage protein. / Schuler, Mary A.; Ladin, Beth F.; Pollaco, Joseph C.; Freyer, Gregory; Beachy, Roger N.

In: Nucleic acids research, Vol. 10, No. 24, 20.12.1982, p. 8245-8261.

Research output: Contribution to journalArticle

Schuler, Mary A. ; Ladin, Beth F. ; Pollaco, Joseph C. ; Freyer, Gregory ; Beachy, Roger N. / Structural sequences are conserved in the genes coding for the α, α′ and β-subunits of the soybean 7S seed storage protein. In: Nucleic acids research. 1982 ; Vol. 10, No. 24. pp. 8245-8261.
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