Structural modifications of Methanococcus jannaschii flagellin proteins revealed by proteome analysis

Carol S. Giometti, Sandra L. Tollaksen, Gyorgy Babnigg, Claudia I. Reich, Gary J Olsen, Hanjo Lim, John R. Yates

Research output: Contribution to journalArticle

Abstract

Methanococcus jannaschii is an autotrophic archaeon originally isolated from an oceanic thermal vent. The primary metabolic path-way for energy production in this hyperthermophilic microbe is methanogenesis from H2 and CO2. As an autotroph, M. jannaschii requires only CO2 as a carbon source for synthesizing all necessary biomolecules. Changes in the environmental availability of these molecules can be expected to activate regulatory mechanisms manifested as the up and down regulation of specific genes and the concomitant increase and decrease in abundance of the corresponding proteins. In our analysis of the proteome of M. jannaschii, we have observed significant changes in the abundance of a common subset of predominant proteins in response to reduced H2 concentration, limited ammonium availability and the stage of cell growth (exponential compared with stationary). The masses of tryptic peptides from these proteins match those predicted by M. jannaschii genome open reading frames annotated as flagellin B1 (MJ0891) and flagellin B2 (MJ0892). Multiple proteins with different isoelectric points and molecular weights match each of these proteins and the abundance of these protein variants changes with growth conditions. These data indicate that structural modifications altering both the isoelectric point and size of the M. jannaschii flagellin B1 and B2 proteins occur in response to growth conditions and growth stage of M. jannaschii and further suggest the regulation of M. jannaschii motility through structural modifications of the building blocks of the flagella.

Original languageEnglish (US)
Pages (from-to)207-217
Number of pages11
JournalEuropean Journal of Mass Spectrometry
Volume7
Issue number2
DOIs
StatePublished - Jan 1 2001

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proteome
Flagellin
Proteome
proteins
Proteins
autotrophs
availability
Genes
Availability
locomotion
genome
Vents
vents
Cell growth
Biomolecules
microorganisms
Ammonium Compounds
genes
set theory
peptides

Keywords

  • Archaea
  • Flagellin
  • Methanococcus jannaschii
  • Proteomics

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Spectroscopy

Cite this

Structural modifications of Methanococcus jannaschii flagellin proteins revealed by proteome analysis. / Giometti, Carol S.; Tollaksen, Sandra L.; Babnigg, Gyorgy; Reich, Claudia I.; Olsen, Gary J; Lim, Hanjo; Yates, John R.

In: European Journal of Mass Spectrometry, Vol. 7, No. 2, 01.01.2001, p. 207-217.

Research output: Contribution to journalArticle

Giometti, Carol S. ; Tollaksen, Sandra L. ; Babnigg, Gyorgy ; Reich, Claudia I. ; Olsen, Gary J ; Lim, Hanjo ; Yates, John R. / Structural modifications of Methanococcus jannaschii flagellin proteins revealed by proteome analysis. In: European Journal of Mass Spectrometry. 2001 ; Vol. 7, No. 2. pp. 207-217.
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