@article{0fa945ee880349a2bd17aea53db9dbb6,
title = "Structural insights into photosystem II assembly",
abstract = "Biogenesis of photosystem II (PSII), nature{\textquoteright}s water-splitting catalyst, is assisted by auxiliary proteins that form transient complexes with PSII components to facilitate stepwise assembly events. Using cryo-electron microscopy, we solved the structure of such a PSII assembly intermediate from Thermosynechococcus elongatus at 2.94 {\AA} resolution. It contains three assembly factors (Psb27, Psb28 and Psb34) and provides detailed insights into their molecular function. Binding of Psb28 induces large conformational changes at the PSII acceptor side, which distort the binding pocket of the mobile quinone (QB) and replace the bicarbonate ligand of non-haem iron with glutamate, a structural motif found in reaction centres of non-oxygenic photosynthetic bacteria. These results reveal mechanisms that protect PSII from damage during biogenesis until water splitting is activated. Our structure further demonstrates how the PSII active site is prepared for the incorporation of the Mn4CaO5 cluster, which performs the unique water-splitting reaction.",
author = "Jure Zabret and Stefan Bohn and Schuller, {Sandra K.} and Oliver Arnolds and Madeline M{\"o}ller and Jakob Meier-Credo and Pasqual Liauw and Aaron Chan and Emad Tajkhorshid and Langer, {Julian D.} and Raphael Stoll and Anja Krieger-Liszkay and Engel, {Benjamin D.} and Till Rudack and Schuller, {Jan M.} and Nowaczyk, {Marc M.}",
note = "Funding Information: We thank C. K{\"o}nig, M. V{\"o}lkel and R. Oworah-Nkruma for excellent technical assistance, K. Becker for cloning of the pIVEXPsb28His plasmid, B. Erjavec for preparation of the scheme in Fig. 1 and N. Cox for helpful discussion. J.M.S. is grateful to E. Conti for scientific independence and great mentorship and to J. M. Plitzko and W. Baumeister for access to the cryo-EM infrastructure and early career support. M.M.N. is grateful to his mentor M. R{\"o}gner for generous support. Financial support was provided by the Max Planck Society, the Helmholtz Zentrum M{\"u}nchen, the Deutsche Forschungsgemeinschaft (DFG) Research Unit FOR2092 (grant nos. EN 1194/1-1 to B.D.E. and 836/3-2 to M.M.N.), the DFG priority programme 2002 (grant no. 836/4-1 to M.M.N. and grant no. 3542/1-1 to J.D.L.), National Institutes of Health (NIH) grant no. NIH P41-GM104601 (to E.T.) and an Emmy-Noether fellowship (SCHU 3364/1-1 to J.M.S). A.K.-L. was supported by the LabEx Saclay Plant Sciences-SPS (grant no. ANR-10-LABX-0040-SPS) and the French Infrastructure for Integrated Structural Biology (FRISBI; grant no. ANR-10-INSB-05). R.S. gratefully acknowledges support from the DFG (grant nos. INST 213/757-1 FUGG and INST 213/843-1 FUGG). Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2021",
month = apr,
doi = "10.1038/s41477-021-00895-0",
language = "English (US)",
volume = "7",
pages = "524--538",
journal = "Nature plants",
issn = "2055-026X",
publisher = "Palgrave Macmillan",
number = "4",
}