Abstract
Sugarcane exemplifies many challenges associated with genetic and genomic analysis of angiosperms, being a recently formed autopolyploid with a large genome, and with its most economically important forms being aneuploid interspecific hybrids. Despite nearly two decades of vigorous activity, current genetic maps remain “incomplete” (with some chromosomes/segments lacking informative sequence-tagged polymorphism) and physical mapping tools are of insufficient depth to cover each allele in any one genotype. Nonetheless, considerable advances have been made by reduced-representation sequencing of cDNA and repetitive DNA. The virtually-complete sequencing of a close relative (sorghum) provides a valuable framework for deducing the probable arrangement of much of the sugarcane genome. Refined sequencing strategies and rapidly dropping costs enhance the likelihood that one or more sugarcane genomes will be sequenced in the near future, and the worldwide sugarcane community continues to discuss various possible sequencing strategies involving different cost levels, different genotypes, and with different expected outcomes. A major contribution is expected from sugarcane genomics regarding the understanding of allelic variation and expression profile in such a complex genomic context. A singular opportunity in the postgenomic era for sugarcane will be to reveal the early events in the adaptation of a genome to the duplicated (polyploid) state, and how this adaptation might relate to productivity of biomass and specific metabolites such as sucrose.
| Original language | English (US) |
|---|---|
| Title of host publication | Genetics, Genomics and Breeding of Sugarcane |
| Publisher | CRC Press |
| Pages | 149-165 |
| Number of pages | 17 |
| ISBN (Electronic) | 9781439848609 |
| ISBN (Print) | 9781578086849 |
| DOIs | |
| State | Published - Jan 1 2010 |
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Keywords
- Autopolyploidy
- Biomass
- C4 photosynthesis
- Comparative genomics
- Sucrose
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Engineering(all)
Cite this
Structural genomics and genome sequencing. / Paterson, Andrew H.; Souza, Glaucia; Van Sluys, Marie Anne; Ming, Ray R; D’Hont, Angelique.
Genetics, Genomics and Breeding of Sugarcane. CRC Press, 2010. p. 149-165.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Structural genomics and genome sequencing
AU - Paterson, Andrew H.
AU - Souza, Glaucia
AU - Van Sluys, Marie Anne
AU - Ming, Ray R
AU - D’Hont, Angelique
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Sugarcane exemplifies many challenges associated with genetic and genomic analysis of angiosperms, being a recently formed autopolyploid with a large genome, and with its most economically important forms being aneuploid interspecific hybrids. Despite nearly two decades of vigorous activity, current genetic maps remain “incomplete” (with some chromosomes/segments lacking informative sequence-tagged polymorphism) and physical mapping tools are of insufficient depth to cover each allele in any one genotype. Nonetheless, considerable advances have been made by reduced-representation sequencing of cDNA and repetitive DNA. The virtually-complete sequencing of a close relative (sorghum) provides a valuable framework for deducing the probable arrangement of much of the sugarcane genome. Refined sequencing strategies and rapidly dropping costs enhance the likelihood that one or more sugarcane genomes will be sequenced in the near future, and the worldwide sugarcane community continues to discuss various possible sequencing strategies involving different cost levels, different genotypes, and with different expected outcomes. A major contribution is expected from sugarcane genomics regarding the understanding of allelic variation and expression profile in such a complex genomic context. A singular opportunity in the postgenomic era for sugarcane will be to reveal the early events in the adaptation of a genome to the duplicated (polyploid) state, and how this adaptation might relate to productivity of biomass and specific metabolites such as sucrose.
AB - Sugarcane exemplifies many challenges associated with genetic and genomic analysis of angiosperms, being a recently formed autopolyploid with a large genome, and with its most economically important forms being aneuploid interspecific hybrids. Despite nearly two decades of vigorous activity, current genetic maps remain “incomplete” (with some chromosomes/segments lacking informative sequence-tagged polymorphism) and physical mapping tools are of insufficient depth to cover each allele in any one genotype. Nonetheless, considerable advances have been made by reduced-representation sequencing of cDNA and repetitive DNA. The virtually-complete sequencing of a close relative (sorghum) provides a valuable framework for deducing the probable arrangement of much of the sugarcane genome. Refined sequencing strategies and rapidly dropping costs enhance the likelihood that one or more sugarcane genomes will be sequenced in the near future, and the worldwide sugarcane community continues to discuss various possible sequencing strategies involving different cost levels, different genotypes, and with different expected outcomes. A major contribution is expected from sugarcane genomics regarding the understanding of allelic variation and expression profile in such a complex genomic context. A singular opportunity in the postgenomic era for sugarcane will be to reveal the early events in the adaptation of a genome to the duplicated (polyploid) state, and how this adaptation might relate to productivity of biomass and specific metabolites such as sucrose.
KW - Autopolyploidy
KW - Biomass
KW - C4 photosynthesis
KW - Comparative genomics
KW - Sucrose
UR - http://www.scopus.com/inward/record.url?scp=79954969597&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79954969597&partnerID=8YFLogxK
U2 - 10.1201/EBK1578086849
DO - 10.1201/EBK1578086849
M3 - Chapter
AN - SCOPUS:79954969597
SN - 9781578086849
SP - 149
EP - 165
BT - Genetics, Genomics and Breeding of Sugarcane
PB - CRC Press
ER -