TY - JOUR
T1 - Structural Basis for Enzymatic Off-Loading of Hybrid Polyketides by Dieckmann Condensation
AU - Cogan, Dillon P.
AU - Ly, Joseph
AU - Nair, Satish K.
N1 - Funding Information:
This work was supported in part by grants from the NIGMS to S.K.N.
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/10/16
Y1 - 2020/10/16
N2 - While several bioactive natural products that contain tetramate or pyridone heterocycles have been described, information on the enzymology underpinning these functionalities has been limited. Here we biochemically characterize an off-loading Dieckmann cyclase, NcmC, that installs the tetramate headgroup in nocamycin, a hybrid polyketide/nonribosomal peptide natural product. Crystal structures of the enzyme (1.6 Å) and its covalent complex with the epoxide cerulenin (1.6 Å) guide additional structure-based mutagenesis and product-profile analyses. Our results offer mechanistic insights into how the conserved thioesterase-like scaffold has been adapted to perform a new chemical reaction, namely, heterocyclization. Additional bioinformatics combined with docking and modeling identifies likely candidates for heterocycle formation in underexplored gene clusters and uncovers a modular basis of substrate recognition by the two subdomains of these Dieckmann cyclases.
AB - While several bioactive natural products that contain tetramate or pyridone heterocycles have been described, information on the enzymology underpinning these functionalities has been limited. Here we biochemically characterize an off-loading Dieckmann cyclase, NcmC, that installs the tetramate headgroup in nocamycin, a hybrid polyketide/nonribosomal peptide natural product. Crystal structures of the enzyme (1.6 Å) and its covalent complex with the epoxide cerulenin (1.6 Å) guide additional structure-based mutagenesis and product-profile analyses. Our results offer mechanistic insights into how the conserved thioesterase-like scaffold has been adapted to perform a new chemical reaction, namely, heterocyclization. Additional bioinformatics combined with docking and modeling identifies likely candidates for heterocycle formation in underexplored gene clusters and uncovers a modular basis of substrate recognition by the two subdomains of these Dieckmann cyclases.
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U2 - 10.1021/acschembio.0c00579
DO - 10.1021/acschembio.0c00579
M3 - Article
C2 - 33017142
AN - SCOPUS:85093538536
SN - 1554-8929
VL - 15
SP - 2783
EP - 2791
JO - ACS chemical biology
JF - ACS chemical biology
IS - 10
ER -