Abstract

A significant amount of the data collected by cell biologists and tissue engineers relies on invasive imaging techniques to visualize dynamic structural and functional properties in engineered tissues. We report the use of optical coherence tomography and the comparative use of confocal microscopy to nondestructively and noninvasively monitor the structural and functional characteristics of three-dimensional engineered tissues over time. The engineered tissue model is composed of chitosan scaffolds and fibroblasts transfected with vinculin fused to green fluorescent protein. We image the developmental process of engineered tissues from changes of tissue microarchitecture to cell-matrix adhesions in three dimensions. These findings demonstrate the potential for optical coherence tomography in applications in cell and tissue biology, tissue engineering, and drug discovery.

Original languageEnglish (US)
Pages (from-to)1747-1756
Number of pages10
JournalTissue Engineering
Volume10
Issue number11-12
DOIs
StatePublished - Jan 1 2004

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Tissue
Imaging techniques
Optical tomography
Confocal microscopy
Structural dynamics
Fibroblasts
Chitosan
Tissue engineering
Scaffolds
Adhesion
Proteins
Engineers

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Cell Biology

Cite this

Structural and functional optical imaging of three-dimensional engineered tissue development. / Tan, Wei; Sendemir-Urkmez, Aylin; Fahrner, Lester J.; Jamison, Russell; Leckband, Deborah; Boppart, Stephen A.

In: Tissue Engineering, Vol. 10, No. 11-12, 01.01.2004, p. 1747-1756.

Research output: Contribution to journalArticle

Tan, Wei ; Sendemir-Urkmez, Aylin ; Fahrner, Lester J. ; Jamison, Russell ; Leckband, Deborah ; Boppart, Stephen A. / Structural and functional optical imaging of three-dimensional engineered tissue development. In: Tissue Engineering. 2004 ; Vol. 10, No. 11-12. pp. 1747-1756.
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