Structural and Biochemical Studies of a Biocatalyst for the Enzymatic Production of Wax Esters

Nektaria Petronikolou, Satish K. Nair

Research output: Contribution to journalArticlepeer-review

Abstract

Wax esters are high-value products whose enzymatic synthesis is of increasing biotechnological interest. The fabrication of cell factories that mass produce wax esters may provide a facile route toward a sustainable, and environmentally friendly, approach to a large-scale process for this commodity chemical. An expedient route for wax ester biocatalysis may be facilitated by the action of enzymes termed wax ester synthases/diacylglycerol acyltransferases (WS/DGAT), which produce wax esters using fatty acids and alcohols as a precursor. In this work, we report the structure for a member of the WS/DGAT superfamily. The structural data in conjunction with bioinformatics and mutational analyses allowed us to identify the substrate-binding pockets, as well as residues that may be important for catalysis. Using this information as a guide, we generated a mutant with preference toward shorter acyl substrates. This study demonstrates the efficacy of a structure-guided engineering effort toward a WS/DGAT variant with preference toward wax esters of desired lengths.

Original languageEnglish (US)
Pages (from-to)6334-6344
Number of pages11
JournalACS Catalysis
Volume8
Issue number7
DOIs
StatePublished - Jul 6 2018

Keywords

  • Marinobacter aquaeolei VT8
  • WS/DGAT
  • biodiesel
  • crystal structure
  • diacylglycerol acyltransferase
  • wax ester synthase
  • wax esters

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry

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