Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging: Melanoma progression as an example

Rohit Bhargava, Rong Kong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Engineered tissue represents a convenient path to providing models for imaging and disease progression. The use of these models or phantoms is becoming increasingly prevalent. While structural characterization of these systems is well-documented, a combination of biochemical and structural knowledge is often helpful. Fourier transform infrared (FTIR) spectroscopic imaging is a rapidly emerging technique that combines the molecular selectivity of spectroscopy with the spatial specificity of optical microscopy. Here, we report on the application of FTIR spectroscopic for analysis of a melanoma model in engineered skin. We first characterize the biochemical properties, consistency and spectral changes in different layers of growing skin. Results provide simple indices for monitoring tissue consistency and reproducibility as a function of time. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. FTIR images indicate holistic chemical changes during the tumor growth, allowing for the development of automated pathology protocols. FTIR imaging being non-destructive, further, samples remain entirely compatible with downstream tissue processing or staining. We specifically examined the correlation of structural changes, molecular content and reproducibility of the model systems. The development of analysis, integrating spectroscopy, imaging and computation will allow for quality control and standardization of both the structural and biochemical properties of tissue phantoms.

Original languageEnglish (US)
Title of host publicationDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
DOIs
StatePublished - Apr 21 2008
EventDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue - San Jose, CA, United States
Duration: Jan 19 2008Jan 21 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6870
ISSN (Print)1605-7422

Other

OtherDesign and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue
CountryUnited States
CitySan Jose, CA
Period1/19/081/21/08

Fingerprint

Fourier transforms
Tissue
Infrared radiation
Imaging techniques
Tumors
Skin
Spectroscopy
Infrared imaging
Pathology
Standardization
Optical microscopy
Quality control
Monitoring
Processing

Keywords

  • Disease model
  • Fourier transform infrared
  • FT-IR spectroscopy
  • Imaging
  • Melanoma
  • Microscopy
  • Skin
  • Tissue engineering

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Bhargava, R., & Kong, R. (2008). Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging: Melanoma progression as an example. In Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue [687004] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6870). https://doi.org/10.1117/12.762511

Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging : Melanoma progression as an example. / Bhargava, Rohit; Kong, Rong.

Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue. 2008. 687004 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6870).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Bhargava, R & Kong, R 2008, Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging: Melanoma progression as an example. in Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue., 687004, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6870, Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue, San Jose, CA, United States, 1/19/08. https://doi.org/10.1117/12.762511
Bhargava R, Kong R. Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging: Melanoma progression as an example. In Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue. 2008. 687004. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.762511
Bhargava, Rohit ; Kong, Rong. / Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging : Melanoma progression as an example. Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue. 2008. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
@inproceedings{f640b20d87534a3e835e1c3cd93a9799,
title = "Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging: Melanoma progression as an example",
abstract = "Engineered tissue represents a convenient path to providing models for imaging and disease progression. The use of these models or phantoms is becoming increasingly prevalent. While structural characterization of these systems is well-documented, a combination of biochemical and structural knowledge is often helpful. Fourier transform infrared (FTIR) spectroscopic imaging is a rapidly emerging technique that combines the molecular selectivity of spectroscopy with the spatial specificity of optical microscopy. Here, we report on the application of FTIR spectroscopic for analysis of a melanoma model in engineered skin. We first characterize the biochemical properties, consistency and spectral changes in different layers of growing skin. Results provide simple indices for monitoring tissue consistency and reproducibility as a function of time. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. FTIR images indicate holistic chemical changes during the tumor growth, allowing for the development of automated pathology protocols. FTIR imaging being non-destructive, further, samples remain entirely compatible with downstream tissue processing or staining. We specifically examined the correlation of structural changes, molecular content and reproducibility of the model systems. The development of analysis, integrating spectroscopy, imaging and computation will allow for quality control and standardization of both the structural and biochemical properties of tissue phantoms.",
keywords = "Disease model, Fourier transform infrared, FT-IR spectroscopy, Imaging, Melanoma, Microscopy, Skin, Tissue engineering",
author = "Rohit Bhargava and Rong Kong",
year = "2008",
month = "4",
day = "21",
doi = "10.1117/12.762511",
language = "English (US)",
isbn = "9780819470454",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue",

}

TY - GEN

T1 - Structural and biochemical characterization of engineered tissue using FTIR spectroscopic imaging

T2 - Melanoma progression as an example

AU - Bhargava, Rohit

AU - Kong, Rong

PY - 2008/4/21

Y1 - 2008/4/21

N2 - Engineered tissue represents a convenient path to providing models for imaging and disease progression. The use of these models or phantoms is becoming increasingly prevalent. While structural characterization of these systems is well-documented, a combination of biochemical and structural knowledge is often helpful. Fourier transform infrared (FTIR) spectroscopic imaging is a rapidly emerging technique that combines the molecular selectivity of spectroscopy with the spatial specificity of optical microscopy. Here, we report on the application of FTIR spectroscopic for analysis of a melanoma model in engineered skin. We first characterize the biochemical properties, consistency and spectral changes in different layers of growing skin. Results provide simple indices for monitoring tissue consistency and reproducibility as a function of time. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. FTIR images indicate holistic chemical changes during the tumor growth, allowing for the development of automated pathology protocols. FTIR imaging being non-destructive, further, samples remain entirely compatible with downstream tissue processing or staining. We specifically examined the correlation of structural changes, molecular content and reproducibility of the model systems. The development of analysis, integrating spectroscopy, imaging and computation will allow for quality control and standardization of both the structural and biochemical properties of tissue phantoms.

AB - Engineered tissue represents a convenient path to providing models for imaging and disease progression. The use of these models or phantoms is becoming increasingly prevalent. While structural characterization of these systems is well-documented, a combination of biochemical and structural knowledge is often helpful. Fourier transform infrared (FTIR) spectroscopic imaging is a rapidly emerging technique that combines the molecular selectivity of spectroscopy with the spatial specificity of optical microscopy. Here, we report on the application of FTIR spectroscopic for analysis of a melanoma model in engineered skin. We first characterize the biochemical properties, consistency and spectral changes in different layers of growing skin. Results provide simple indices for monitoring tissue consistency and reproducibility as a function of time. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. FTIR images indicate holistic chemical changes during the tumor growth, allowing for the development of automated pathology protocols. FTIR imaging being non-destructive, further, samples remain entirely compatible with downstream tissue processing or staining. We specifically examined the correlation of structural changes, molecular content and reproducibility of the model systems. The development of analysis, integrating spectroscopy, imaging and computation will allow for quality control and standardization of both the structural and biochemical properties of tissue phantoms.

KW - Disease model

KW - Fourier transform infrared

KW - FT-IR spectroscopy

KW - Imaging

KW - Melanoma

KW - Microscopy

KW - Skin

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=42149088875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149088875&partnerID=8YFLogxK

U2 - 10.1117/12.762511

DO - 10.1117/12.762511

M3 - Conference contribution

AN - SCOPUS:42149088875

SN - 9780819470454

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurements of Tissue

ER -