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Structural analysis of the rabbit TNF locus, containing the genes encoding TNF-β(lymphotoxin) and TNF-α(tumor necrosis factor)

  • A. N. Shakhov
  • , D. V. Kuprash
  • , M. M. Azizov
  • , C. V. Jongeneel
  • , S. A. Nedospasov

Research output: Contribution to journalArticlepeer-review

Abstract

The genes coding for 20-kDa lymphotoxin (TNF-β) and tumor necrosis factor (TNF-α) have been cloned from a rabbit genomic library. The two genes are tandemly arranged and separated by only 1 kb of DNA, as previously observed in human and mouse genomes. We have sequenced the entire rabbit lymphotoxin-encoding gene and inferred the primary structure of rabbit TNF-β, whose cDNA is not yet cloned. We also analysed the upstream sequences of the rabbit TNF-β and TNF-α genes and identified a number of potential binding sites for known nuclear transcription factors, and in particular several putative κB-type sequences.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalGene
Volume95
Issue number2
DOIs
StatePublished - Nov 15 1990
Externally publishedYes

Keywords

  • Recombinant DNA
  • cytokines
  • gene duplication
  • transcription factors
  • upstream regions

ASJC Scopus subject areas

  • Genetics

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