Strategy for room temperature spectroscopy of single molecules

T. Ha, D. S. Chemla, Th Enderle, S. Weiss

Research output: Contribution to journalReview article

Abstract

Laser-induced fluorescence (LIF) from single molecules at room temperature displays rich dynamics of reversible and irreversible transitions within the photodestruction lifetime of the molecule. In contrast to ensemble studies, these transitions are directly resolved when the fluorescence signal is monitored in time. We present a strategy and describe the instrumentation needed for spectroscopic studies on a large number of individual molecules. It is based on a computer controlled optical system which automatically and rapidly positions single molecules in the excitation volume of a confocal microscope and subsequently performs spectroscopic measurements. Examples for such spectroscopies are presented.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalBioimaging
Volume5
Issue number3
DOIs
StatePublished - Jan 1 1997

Keywords

  • Laser-induced fluorescence
  • Single molecule spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging

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