Strain-specific interaction of a GII.10 Norovirus with HBGAs

Miao Jin, Ming Tan, Ming Xia, Chao Wei, Pengwei Huang, Leyi Wang, Weiming Zhong, Zhaojun Duan, Xi Jiang

Research output: Contribution to journalArticlepeer-review

Abstract

Noroviruses (NoVs), an important cause of gastroenteritis in humans, recognize human histo-blood group antigens (HBGAs) as receptors. The crystal structures of the protruding (P) domain of a GII.10 NoV (Vietnam 026) in complex with various HBGA oligosaccharides were elucidated. However, the HBGA binding profile of this virus remains unknown. In this study, we determined the saliva and oligosaccharide binding profiles of this virus and the roles of amino acids that are involved in HBGA binding. Our data showed that Vietnam 026 bound to all ABO secretor and non-secretor saliva with clear signals detected by monoclonal antibodies against H3, H1, Ley, Lea and sialyl Lea. Mutagenesis study confirmed the binding site determined by the crystallography study, in which single mutations wiped out the binding function. We also identified amino acids surrounding the central binding pocket that may participate in the binding by affecting the HBGA binding specificity of the GII.10 NoV.

Original languageEnglish (US)
Pages (from-to)386-394
Number of pages9
JournalVirology
Volume476
DOIs
StatePublished - Feb 1 2015
Externally publishedYes

Keywords

  • Acute gastroenteritis
  • Human histo-blood group antigens
  • Mutagenesis
  • Noroviruses
  • Oligosaccharide
  • Saliva

ASJC Scopus subject areas

  • Virology

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