Strain development by whole-cell directed evolution

Tong Si, Jiazhang Lian, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Due to limited knowledge of complicated cellular networks, directed evolution has played critical roles in strain improvement, especially for complex traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapter, we summarize recent technological advances and applications of directed evolution in strain development, focusing on the efforts for accelerating evolution workflows, expanding the range of target phenotypes, and facilitating mechanistic understanding of evolved mutations.

Original languageEnglish (US)
Title of host publicationDirected Enzyme Evolution
Subtitle of host publicationAdvances and Applications
PublisherSpringer International Publishing
Pages173-200
Number of pages28
ISBN (Electronic)9783319504131
ISBN (Print)9783319504117
DOIs
StatePublished - Jan 1 2017

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Chemical Engineering(all)
  • Medicine(all)

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  • Cite this

    Si, T., Lian, J., & Zhao, H. (2017). Strain development by whole-cell directed evolution. In Directed Enzyme Evolution: Advances and Applications (pp. 173-200). Springer International Publishing. https://doi.org/10.1007/978-3-319-50413-1_7