Strain development by whole-cell directed evolution

Tong Si, Jiazhang Lian, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Due to limited knowledge of complicated cellular networks, directed evolution has played critical roles in strain improvement, especially for complex traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapter, we summarize recent technological advances and applications of directed evolution in strain development, focusing on the efforts for accelerating evolution workflows, expanding the range of target phenotypes, and facilitating mechanistic understanding of evolved mutations.

Original languageEnglish (US)
Title of host publicationDirected Enzyme Evolution
Subtitle of host publicationAdvances and Applications
PublisherSpringer International Publishing
Pages173-200
Number of pages28
ISBN (Electronic)9783319504131
ISBN (Print)9783319504117
DOIs
StatePublished - Jan 1 2017

Fingerprint

Phenotype
Workflow
Screening
Mutation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Chemical Engineering(all)
  • Medicine(all)

Cite this

Si, T., Lian, J., & Zhao, H. (2017). Strain development by whole-cell directed evolution. In Directed Enzyme Evolution: Advances and Applications (pp. 173-200). Springer International Publishing. https://doi.org/10.1007/978-3-319-50413-1_7

Strain development by whole-cell directed evolution. / Si, Tong; Lian, Jiazhang; Zhao, Huimin.

Directed Enzyme Evolution: Advances and Applications. Springer International Publishing, 2017. p. 173-200.

Research output: Chapter in Book/Report/Conference proceedingChapter

Si, T, Lian, J & Zhao, H 2017, Strain development by whole-cell directed evolution. in Directed Enzyme Evolution: Advances and Applications. Springer International Publishing, pp. 173-200. https://doi.org/10.1007/978-3-319-50413-1_7
Si T, Lian J, Zhao H. Strain development by whole-cell directed evolution. In Directed Enzyme Evolution: Advances and Applications. Springer International Publishing. 2017. p. 173-200 https://doi.org/10.1007/978-3-319-50413-1_7
Si, Tong ; Lian, Jiazhang ; Zhao, Huimin. / Strain development by whole-cell directed evolution. Directed Enzyme Evolution: Advances and Applications. Springer International Publishing, 2017. pp. 173-200
@inbook{5604327879964a33bc2d431b92920a33,
title = "Strain development by whole-cell directed evolution",
abstract = "Due to limited knowledge of complicated cellular networks, directed evolution has played critical roles in strain improvement, especially for complex traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapter, we summarize recent technological advances and applications of directed evolution in strain development, focusing on the efforts for accelerating evolution workflows, expanding the range of target phenotypes, and facilitating mechanistic understanding of evolved mutations.",
author = "Tong Si and Jiazhang Lian and Huimin Zhao",
year = "2017",
month = "1",
day = "1",
doi = "10.1007/978-3-319-50413-1_7",
language = "English (US)",
isbn = "9783319504117",
pages = "173--200",
booktitle = "Directed Enzyme Evolution",
publisher = "Springer International Publishing",

}

TY - CHAP

T1 - Strain development by whole-cell directed evolution

AU - Si, Tong

AU - Lian, Jiazhang

AU - Zhao, Huimin

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Due to limited knowledge of complicated cellular networks, directed evolution has played critical roles in strain improvement, especially for complex traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapter, we summarize recent technological advances and applications of directed evolution in strain development, focusing on the efforts for accelerating evolution workflows, expanding the range of target phenotypes, and facilitating mechanistic understanding of evolved mutations.

AB - Due to limited knowledge of complicated cellular networks, directed evolution has played critical roles in strain improvement, especially for complex traits with hundreds of genetic determinants and for organisms with few genetic tools. Directed evolution mimics natural evolution in the laboratory via iterative cycles of diversity generation and functional selection or screening to isolate evolved mutants with desirable phenotypes. In this chapter, we summarize recent technological advances and applications of directed evolution in strain development, focusing on the efforts for accelerating evolution workflows, expanding the range of target phenotypes, and facilitating mechanistic understanding of evolved mutations.

UR - http://www.scopus.com/inward/record.url?scp=85019898853&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019898853&partnerID=8YFLogxK

U2 - 10.1007/978-3-319-50413-1_7

DO - 10.1007/978-3-319-50413-1_7

M3 - Chapter

AN - SCOPUS:85019898853

SN - 9783319504117

SP - 173

EP - 200

BT - Directed Enzyme Evolution

PB - Springer International Publishing

ER -