Abstract
We describe a simple yet highly effective optimization strategy for SPINAL-64 1H decoupling conditions for magic-angle spinning solid-state NMR. With adjustment of the phase angles in a coupled manner, the optimal conditions resulting from three parameter optimizations can be determined with adjustment of a single phase. Notably, echo T2 relaxation times for 13C and 15N show significant enhancement (up to 64%), relative to the previous described SPINAL-64 conditions, under the moderate 1H decoupling levels (60-100 kHz) and MAS rate (13.3 kHz) commonly employed for high-resolution SSNMR spectroscopy of proteins. Additionally, we also investigated the effect at higher spinning rate (33.3 kHz) and compared the results with other 1H decoupling schemes (TPPM, XiX), as well as SPINAL-64 with the originally reported optimal values.
Original language | English (US) |
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Pages (from-to) | 131-135 |
Number of pages | 5 |
Journal | Journal of Magnetic Resonance |
Volume | 209 |
Issue number | 2 |
DOIs | |
State | Published - Apr 2011 |
Keywords
- Magic-angle spinning
- Proton decoupling
- SPINAL-64
- Solid-state MMR
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Nuclear and High Energy Physics
- Condensed Matter Physics