Stepwise translocation of nucleic acid motors

Sua Myong, Taekjip Ha

Research output: Contribution to journalReview article

Abstract

Recent single molecule studies have made a significant contribution to the understanding of the molecular mechanism involved in the movement of motor proteins which process DNA and RNA. Measurement of stepsize in two disparate motors, NS3 helicase and ribosome both revealed 3-bp steps, which consist of three hidden substeps. Combined with previous structural studies, NS3 is likely taking a single nucleotide step of translocation coupled to one ATP binding event and this mode may be conserved in multitude of helicases. Such a stepwise translocation movement appears to occur through main contacts with the phosphate backbone. Double stranded RNA and DNA motor, RIG-I and φ{symbol}29, respectively, showed translocation on a duplex while tracking exclusively a single strand of RNA or DNA in a directional manner, 5′-3′ in both cases. Spontaneous dynamics displayed by ribosome ratcheting and SSB (single stranded DNA binding protein) diffusing on DNA were rectified by interacting cofactors and proteins, EF-G and RecA, respectively.

Original languageEnglish (US)
Pages (from-to)121-127
Number of pages7
JournalCurrent Opinion in Structural Biology
Volume20
Issue number1
DOIs
StatePublished - Feb 1 2010

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Nucleic Acids
DNA
Ribosomes
Peptide Elongation Factor G
RNA
Double-Stranded RNA
DNA-Binding Proteins
Proteins
Nucleotides
Adenosine Triphosphate
Phosphates

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Stepwise translocation of nucleic acid motors. / Myong, Sua; Ha, Taekjip.

In: Current Opinion in Structural Biology, Vol. 20, No. 1, 01.02.2010, p. 121-127.

Research output: Contribution to journalReview article

Myong, Sua ; Ha, Taekjip. / Stepwise translocation of nucleic acid motors. In: Current Opinion in Structural Biology. 2010 ; Vol. 20, No. 1. pp. 121-127.
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