Staphylococcal superantigens cause lethal pulmonary disease in rabbits

Kristi L. Strandberg, Jessica H. Rotschafer, Sara M. Vetter, Rebecca A. Buonpane, David M Kranz, Patrick M. Schlievert

Research output: Contribution to journalArticle

Abstract

Background. The Centers for Disease Control and Prevention (CDC) and others reported that methicillin-resistant S. aureus (MRSA) are significant causes of serious human infections, including pulmonary illnesses. We investigated the role played by superantigens in lung-associated lethal illness in rabbits. Methods. A rabbit model was established to investigate the potential role played by superantigens, staphylococcal enterotoxin B (SEB), staphylococcal enterotoxin C (SEC), and toxic shock syndrome toxin-1 (TSST-1). Rabbits received intrabronchial community-associated (CA) MRSA strains USA200 (TSST-1 +), MW2 (SEC+), c99-529 (SEB+), or purified superantigens. Some rabbits were preimmunized against superantigens or treated with soluble high-affinity T cell receptors (Vβ-TCR) to neutralize SEB and then challenged intrabronchially with CA-MRSA or superantigens. Results. Rabbits challenged with CA-MRSA or superantigens developed fatal, pulmonary illnesses. Animals preimmunized against purified superantigens, or treated passively with Vβ-TCRs and then challenged with CA-MRSA or superantigens, survived. Lung histological analysis indicated that nonimmune animals developed lesions consistent with necrotizing pneumonia after challenge with CA-MRSA or purified superantigens. Superantigenimmune animals or animals treated with soluble Vβ-TCRs did not develop pulmonary lesions. Conclusions. Superantigens contribute to lethal pulmonary illnesses due to CA-MRSA; preexisting immunity to superantigens prevents lethality. Administration of high-affinity Vβ-TCR with specificity for SEB to nonimmune animals protects from lethal pulmonary illness resulting from SEB+ CA-MRSA and SEB.

Original languageEnglish (US)
Pages (from-to)1690-1697
Number of pages8
JournalJournal of Infectious Diseases
Volume202
Issue number11
DOIs
StatePublished - Dec 1 2010

Fingerprint

Superantigens
Lung Diseases
Methicillin Resistance
Rabbits
Lung
Centers for Disease Control and Prevention (U.S.)
T-Cell Antigen Receptor
staphylococcal enterotoxin B
Immunity

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Strandberg, K. L., Rotschafer, J. H., Vetter, S. M., Buonpane, R. A., Kranz, D. M., & Schlievert, P. M. (2010). Staphylococcal superantigens cause lethal pulmonary disease in rabbits. Journal of Infectious Diseases, 202(11), 1690-1697. https://doi.org/10.1086/657156

Staphylococcal superantigens cause lethal pulmonary disease in rabbits. / Strandberg, Kristi L.; Rotschafer, Jessica H.; Vetter, Sara M.; Buonpane, Rebecca A.; Kranz, David M; Schlievert, Patrick M.

In: Journal of Infectious Diseases, Vol. 202, No. 11, 01.12.2010, p. 1690-1697.

Research output: Contribution to journalArticle

Strandberg, KL, Rotschafer, JH, Vetter, SM, Buonpane, RA, Kranz, DM & Schlievert, PM 2010, 'Staphylococcal superantigens cause lethal pulmonary disease in rabbits', Journal of Infectious Diseases, vol. 202, no. 11, pp. 1690-1697. https://doi.org/10.1086/657156
Strandberg KL, Rotschafer JH, Vetter SM, Buonpane RA, Kranz DM, Schlievert PM. Staphylococcal superantigens cause lethal pulmonary disease in rabbits. Journal of Infectious Diseases. 2010 Dec 1;202(11):1690-1697. https://doi.org/10.1086/657156
Strandberg, Kristi L. ; Rotschafer, Jessica H. ; Vetter, Sara M. ; Buonpane, Rebecca A. ; Kranz, David M ; Schlievert, Patrick M. / Staphylococcal superantigens cause lethal pulmonary disease in rabbits. In: Journal of Infectious Diseases. 2010 ; Vol. 202, No. 11. pp. 1690-1697.
@article{2da9ccef371543cf8e38ef7d8366930e,
title = "Staphylococcal superantigens cause lethal pulmonary disease in rabbits",
abstract = "Background. The Centers for Disease Control and Prevention (CDC) and others reported that methicillin-resistant S. aureus (MRSA) are significant causes of serious human infections, including pulmonary illnesses. We investigated the role played by superantigens in lung-associated lethal illness in rabbits. Methods. A rabbit model was established to investigate the potential role played by superantigens, staphylococcal enterotoxin B (SEB), staphylococcal enterotoxin C (SEC), and toxic shock syndrome toxin-1 (TSST-1). Rabbits received intrabronchial community-associated (CA) MRSA strains USA200 (TSST-1 +), MW2 (SEC+), c99-529 (SEB+), or purified superantigens. Some rabbits were preimmunized against superantigens or treated with soluble high-affinity T cell receptors (Vβ-TCR) to neutralize SEB and then challenged intrabronchially with CA-MRSA or superantigens. Results. Rabbits challenged with CA-MRSA or superantigens developed fatal, pulmonary illnesses. Animals preimmunized against purified superantigens, or treated passively with Vβ-TCRs and then challenged with CA-MRSA or superantigens, survived. Lung histological analysis indicated that nonimmune animals developed lesions consistent with necrotizing pneumonia after challenge with CA-MRSA or purified superantigens. Superantigenimmune animals or animals treated with soluble Vβ-TCRs did not develop pulmonary lesions. Conclusions. Superantigens contribute to lethal pulmonary illnesses due to CA-MRSA; preexisting immunity to superantigens prevents lethality. Administration of high-affinity Vβ-TCR with specificity for SEB to nonimmune animals protects from lethal pulmonary illness resulting from SEB+ CA-MRSA and SEB.",
author = "Strandberg, {Kristi L.} and Rotschafer, {Jessica H.} and Vetter, {Sara M.} and Buonpane, {Rebecca A.} and Kranz, {David M} and Schlievert, {Patrick M.}",
year = "2010",
month = "12",
day = "1",
doi = "10.1086/657156",
language = "English (US)",
volume = "202",
pages = "1690--1697",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Staphylococcal superantigens cause lethal pulmonary disease in rabbits

AU - Strandberg, Kristi L.

AU - Rotschafer, Jessica H.

AU - Vetter, Sara M.

AU - Buonpane, Rebecca A.

AU - Kranz, David M

AU - Schlievert, Patrick M.

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Background. The Centers for Disease Control and Prevention (CDC) and others reported that methicillin-resistant S. aureus (MRSA) are significant causes of serious human infections, including pulmonary illnesses. We investigated the role played by superantigens in lung-associated lethal illness in rabbits. Methods. A rabbit model was established to investigate the potential role played by superantigens, staphylococcal enterotoxin B (SEB), staphylococcal enterotoxin C (SEC), and toxic shock syndrome toxin-1 (TSST-1). Rabbits received intrabronchial community-associated (CA) MRSA strains USA200 (TSST-1 +), MW2 (SEC+), c99-529 (SEB+), or purified superantigens. Some rabbits were preimmunized against superantigens or treated with soluble high-affinity T cell receptors (Vβ-TCR) to neutralize SEB and then challenged intrabronchially with CA-MRSA or superantigens. Results. Rabbits challenged with CA-MRSA or superantigens developed fatal, pulmonary illnesses. Animals preimmunized against purified superantigens, or treated passively with Vβ-TCRs and then challenged with CA-MRSA or superantigens, survived. Lung histological analysis indicated that nonimmune animals developed lesions consistent with necrotizing pneumonia after challenge with CA-MRSA or purified superantigens. Superantigenimmune animals or animals treated with soluble Vβ-TCRs did not develop pulmonary lesions. Conclusions. Superantigens contribute to lethal pulmonary illnesses due to CA-MRSA; preexisting immunity to superantigens prevents lethality. Administration of high-affinity Vβ-TCR with specificity for SEB to nonimmune animals protects from lethal pulmonary illness resulting from SEB+ CA-MRSA and SEB.

AB - Background. The Centers for Disease Control and Prevention (CDC) and others reported that methicillin-resistant S. aureus (MRSA) are significant causes of serious human infections, including pulmonary illnesses. We investigated the role played by superantigens in lung-associated lethal illness in rabbits. Methods. A rabbit model was established to investigate the potential role played by superantigens, staphylococcal enterotoxin B (SEB), staphylococcal enterotoxin C (SEC), and toxic shock syndrome toxin-1 (TSST-1). Rabbits received intrabronchial community-associated (CA) MRSA strains USA200 (TSST-1 +), MW2 (SEC+), c99-529 (SEB+), or purified superantigens. Some rabbits were preimmunized against superantigens or treated with soluble high-affinity T cell receptors (Vβ-TCR) to neutralize SEB and then challenged intrabronchially with CA-MRSA or superantigens. Results. Rabbits challenged with CA-MRSA or superantigens developed fatal, pulmonary illnesses. Animals preimmunized against purified superantigens, or treated passively with Vβ-TCRs and then challenged with CA-MRSA or superantigens, survived. Lung histological analysis indicated that nonimmune animals developed lesions consistent with necrotizing pneumonia after challenge with CA-MRSA or purified superantigens. Superantigenimmune animals or animals treated with soluble Vβ-TCRs did not develop pulmonary lesions. Conclusions. Superantigens contribute to lethal pulmonary illnesses due to CA-MRSA; preexisting immunity to superantigens prevents lethality. Administration of high-affinity Vβ-TCR with specificity for SEB to nonimmune animals protects from lethal pulmonary illness resulting from SEB+ CA-MRSA and SEB.

UR - http://www.scopus.com/inward/record.url?scp=78649558101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649558101&partnerID=8YFLogxK

U2 - 10.1086/657156

DO - 10.1086/657156

M3 - Article

C2 - 20979456

AN - SCOPUS:78649558101

VL - 202

SP - 1690

EP - 1697

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 11

ER -