Figure Persented: We report the development of a multilayered graphene-Al 2O 3 nanopore platform for the sensitive detection of DNA and DNA-protein complexes. Graphene-Al 2O 3 nanolaminate membranes are formed by sequentially depositing layers of graphene and Al 2O 3, with nanopores being formed in these membranes using an electron-beam sculpting process. The resulting nanopores are highly robust, exhibit low electrical noise (significantly lower than nanopores in pure graphene), are highly sensitive to electrolyte pH at low KCl concentrations (attributed to the high buffer capacity of Al 2O 3), and permit the electrical biasing of the embedded graphene electrode, thereby allowing for three terminal nanopore measurements. In proof-of-principle biomolecule sensing experiments, the folded and unfolded transport of single DNA molecules and RecA-coated DNA complexes could be discerned with high temporal resolution. The process described here also enables nanopore integration with new graphene-based structures, including nanoribbons and nanogaps, for single-molecule DNA sequencing and medical diagnostic applications.

Original languageEnglish (US)
Pages (from-to)441-450
Number of pages10
JournalACS Nano
Issue number1
StatePublished - Jan 24 2012


  • DNA
  • grapheme
  • nanopore
  • sequencing
  • translocation

ASJC Scopus subject areas

  • General Engineering
  • General Materials Science
  • General Physics and Astronomy


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