Stable small quantum dots for synaptic receptor tracking on live neurons

En Cai, Pinghua Ge, Sang Hak Lee, Okunola Jeyifous, Yong Wang, Yanxin Liu, Katie M. Wilson, Sung Jun Lim, Michelle A. Baird, John E. Stone, Kwan Young Lee, Michael W. Davidson, Hee Jung Chung, Klaus Schulten, Andrew M. Smith, William N. Green, Paul R. Selvin

Research output: Contribution to journalArticlepeer-review

Abstract

We developed a coating method to produce functionalized small quantum dots (sQDs), about 9 nmin diameter, that were stable for over a month. We made sQDs in four emission wavelengths, from 527 to 655 nm and with different functional groups. AMPA receptors on live neurons were labeled with sQDs and postsynaptic density proteins were visualized with super-resolution microscopy. Their diffusion behavior indicates that sQDs access the synaptic clefts significantly more often than commercial QDs.

Original languageEnglish (US)
Pages (from-to)12484-12488
Number of pages5
JournalAngewandte Chemie - International Edition
Volume53
Issue number46
DOIs
StatePublished - Nov 10 2014

Keywords

  • AMPA receptors
  • Fluorescent probes
  • Quantum dots
  • Single-molecule studies

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis

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