Stable binding of alternative protein-enriched food matrices with concentrated cranberry bioflavonoids for functional food applications

Mary H. Grace, Ivette Guzman, Diana E. Roopchand, Kristin Moskal, Diana M. Cheng, Natasha Pogrebnyak, Ilya Raskin, Amy Howell, Mary Ann Lila

Research output: Contribution to journalArticlepeer-review

Abstract

Defatted soy flour (DSF), soy protein isolate (SPI), hemp protein isolate (HPI), medium-roast peanut flour (MPF), and pea protein isolate (PPI) stably bind and concentrate cranberry (CB) polyphenols, creating protein/polyphenol- enriched matrices. Proanthocyanidins (PAC) in the enriched matrices ranged from 20.75 mg/g (CB-HPI) to 10.68 mg/g (CB-SPI). Anthocyanins (ANC) ranged from 3.19 mg/g (CB-DSF) to 1.68 mg/g (CB-SPI), whereas total phenolics (TP) ranged from 37.61 mg/g (CB-HPI) to 21.29 mg/g (CB-SPI). LC-MS indicated that the enriched matrices contained all identifiable ANC, PAC, and flavonols present in CB juice. Complexation with SPI stabilized and preserved the integrity of the CB polyphenolic components for at least 15 weeks at 37 C. PAC isolated from enriched matrices demonstrated comparable antiadhesion bioactivity to PAC isolated directly from CB juice (MIC 0.4-0.16 mg/mL), indicating their potential utility for maintenance of urinary tract health. Approximately 1.0 g of polyphenol-enriched matrix delivered the same amount of PAC available in 1 cup (300 mL) of commercial CB juice cocktail, which has been shown clinically to be the prophylactic dose for reducing recurring urinary tract infections. CB-SPI inhibited Gram-positive and Gram-negative bacterial growth. Nutritional and sensory analyses indicated that the targeted CB-matrix combinations have high potential for incorporation in functional food formulations.

Original languageEnglish (US)
Pages (from-to)6856-6864
Number of pages9
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number28
DOIs
StatePublished - Jul 17 2013
Externally publishedYes

Keywords

  • A-type proanthocyanidins
  • Vaccinium macrocarpon
  • antiadhesion
  • antimicrobial
  • polyphenols
  • shelf stability

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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