Stability of RAPD markers for determining cultivar specific DNA profiles in rye (Secale cereale L.)

M. Javed Iqbal, A. Lane Rayburn

Research output: Contribution to journalArticle

Abstract

Cultivar specific DNA profiles in rye were revealed by polymerase chain reaction (PCR) using randomly amplified polymorphic DNA (RAPD) sequences. Ten base primers were used for the amplification of genomic DNA of rye cultivars by PCR. RAPD analysis was found to be reproducible among samples between PCR runs. When amplification profiles of different rye cultivars were compared using various primers, the overall profiles were cultivar specific. However, not all primers revealed polymorphisms. These primers appear to amplify conserved sequences in all rye cultivars. Intracultivar studies were conducted on two of the cultivars. In the cultivar Imperial, no polymorphisms were observed among ten plants analyzed with five primers. In the cultivar Balboa, polymorphisms were observed among fifty plants with four of the ten primers analyzed. Despite the small amount of intracultivar variability, RAPD analysis has the potential to be a rapid and reliable method of cultivar identification in this outcrossing species.

Original languageEnglish (US)
Pages (from-to)215-220
Number of pages6
JournalEuphytica
Volume75
Issue number3
DOIs
StatePublished - Jan 1 1994

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Keywords

  • DNA
  • PCR
  • RAPDs
  • Secale cereale
  • polymorphisms
  • rye
  • stability

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

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