SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum

Changsoo Kim, Dong Zhang, Susan A. Auckland, Lisa K. Rainville, Katrin Jakob, Brent Kronmiller, Erik J. Sacks, Martin Deuter, Andrew H. Paterson

Research output: Contribution to journalArticle

Abstract

We present SSR-based genetic maps from a cross between Miscanthus sacchariflorus Robustus and M. sinensis, the progenitors of the promising cellulosic biofuel feedstock Miscanthus × giganteus. cDNA-derived SSR markers were mapped by the two-way pseudo-testcross model due to the high heterozygosity of each parental species. A total of 261 loci were mapped in M. sacchariflorus, spanning 40 linkage groups and 1,998.8 cM, covering an estimated 72.7% of the genome. For M. sinensis, a total of 303 loci were mapped, forming 23 linkage groups and 2,238.3 cM, covering 84.9% of the genome. The use of cDNA-derived SSR loci permitted alignment of the Miscanthus linkage groups to the sorghum chromosomes, revealing a whole genome duplication affecting the Miscanthus lineage after the divergence of subtribes Sorghinae and Saccharinae, as well as traces of the pan-cereal whole genome duplication. While the present maps provide for many early research needs in this emerging crop, additional markers are also needed to improve map density and to further characterize the structural changes of the Miscanthus genome since its divergence from sorghum and Saccharum.

Original languageEnglish (US)
Pages (from-to)1325-1338
Number of pages14
JournalTheoretical and Applied Genetics
Volume124
Issue number7
DOIs
StatePublished - Jan 24 2012

Fingerprint

Miscanthus sacchariflorus
Miscanthus sinensis
Sorghum
Sorghum (Poaceae)
Miscanthus
Genome
linkage groups
genome
loci
Complementary DNA
Miscanthus giganteus
Saccharum
testcrosses
Biofuels
feedstocks
biofuels
heterozygosity
Chromosomes
chromosomes
crops

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Kim, C., Zhang, D., Auckland, S. A., Rainville, L. K., Jakob, K., Kronmiller, B., ... Paterson, A. H. (2012). SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum. Theoretical and Applied Genetics, 124(7), 1325-1338. https://doi.org/10.1007/s00122-012-1790-1

SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum. / Kim, Changsoo; Zhang, Dong; Auckland, Susan A.; Rainville, Lisa K.; Jakob, Katrin; Kronmiller, Brent; Sacks, Erik J.; Deuter, Martin; Paterson, Andrew H.

In: Theoretical and Applied Genetics, Vol. 124, No. 7, 24.01.2012, p. 1325-1338.

Research output: Contribution to journalArticle

Kim, C, Zhang, D, Auckland, SA, Rainville, LK, Jakob, K, Kronmiller, B, Sacks, EJ, Deuter, M & Paterson, AH 2012, 'SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum', Theoretical and Applied Genetics, vol. 124, no. 7, pp. 1325-1338. https://doi.org/10.1007/s00122-012-1790-1
Kim, Changsoo ; Zhang, Dong ; Auckland, Susan A. ; Rainville, Lisa K. ; Jakob, Katrin ; Kronmiller, Brent ; Sacks, Erik J. ; Deuter, Martin ; Paterson, Andrew H. / SSR-based genetic maps of Miscanthus sinensis and M. sacchariflorus, and their comparison to sorghum. In: Theoretical and Applied Genetics. 2012 ; Vol. 124, No. 7. pp. 1325-1338.
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