SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles

Vidisha Tripathi, David Y. Song, Xinying Zong, Sergey P. Shevtsov, Stephen Hearn, Xiang Dong Fu, Miroslav Dundr, Kannanganattu V. Prasanth

Research output: Contribution to journalArticle

Abstract

The mammalian cell nucleus is compartmentalized into nonmembranous subnuclear domains that regulate key nuclear functions. Nuclear speckles are subnuclear domains that contain pre-mRNA processing factors and noncoding RNAs. Many of the nuclear speckle constituents work in concert to coordinate multiple steps of gene expression, including transcription, pre-mRNA processing and mRNA transport. The mechanism that regulates the formation and maintenance of nuclear speckles in the interphase nucleus is poorly understood. In the present study, we provide evidence for the involvement of nuclear speckle resident proteins and RNA components in the organization of nuclear speckles. SR-family splicing factors and their binding partner, long noncoding metastasis-associated lung adenocarcinoma transcript 1 RNA, can nucleate the assembly of nuclear speckles in the interphase nucleus. Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and activity of other SR proteins. Furthermore, on a stably integrated reporter gene locus, we demonstrate the role of SRSF1 in RNA polymerase II-mediated transcription. Our results suggest that SR proteins mediate the assembly of nuclear speckles and regulate gene expression by influencing both transcriptional and posttranscriptional activities within the cell nucleus.

Original languageEnglish (US)
Pages (from-to)3694-3706
Number of pages13
JournalMolecular biology of the cell
Volume23
Issue number18
DOIs
StatePublished - Sep 15 2012

Fingerprint

RNA Precursors
Interphase
Cell Nucleus
RNA
Gene Expression
Untranslated RNA
Proteins
RNA Polymerase II
Reporter Genes
Maintenance
Neoplasm Metastasis
Messenger RNA
RNA Splicing Factors

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles. / Tripathi, Vidisha; Song, David Y.; Zong, Xinying; Shevtsov, Sergey P.; Hearn, Stephen; Fu, Xiang Dong; Dundr, Miroslav; Prasanth, Kannanganattu V.

In: Molecular biology of the cell, Vol. 23, No. 18, 15.09.2012, p. 3694-3706.

Research output: Contribution to journalArticle

Tripathi, V, Song, DY, Zong, X, Shevtsov, SP, Hearn, S, Fu, XD, Dundr, M & Prasanth, KV 2012, 'SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles', Molecular biology of the cell, vol. 23, no. 18, pp. 3694-3706. https://doi.org/10.1091/mbc.E12-03-0206
Tripathi V, Song DY, Zong X, Shevtsov SP, Hearn S, Fu XD et al. SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles. Molecular biology of the cell. 2012 Sep 15;23(18):3694-3706. https://doi.org/10.1091/mbc.E12-03-0206
Tripathi, Vidisha ; Song, David Y. ; Zong, Xinying ; Shevtsov, Sergey P. ; Hearn, Stephen ; Fu, Xiang Dong ; Dundr, Miroslav ; Prasanth, Kannanganattu V. / SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles. In: Molecular biology of the cell. 2012 ; Vol. 23, No. 18. pp. 3694-3706.
@article{c823d0fd5d794859ad8f66f0fb450d40,
title = "SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles",
abstract = "The mammalian cell nucleus is compartmentalized into nonmembranous subnuclear domains that regulate key nuclear functions. Nuclear speckles are subnuclear domains that contain pre-mRNA processing factors and noncoding RNAs. Many of the nuclear speckle constituents work in concert to coordinate multiple steps of gene expression, including transcription, pre-mRNA processing and mRNA transport. The mechanism that regulates the formation and maintenance of nuclear speckles in the interphase nucleus is poorly understood. In the present study, we provide evidence for the involvement of nuclear speckle resident proteins and RNA components in the organization of nuclear speckles. SR-family splicing factors and their binding partner, long noncoding metastasis-associated lung adenocarcinoma transcript 1 RNA, can nucleate the assembly of nuclear speckles in the interphase nucleus. Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and activity of other SR proteins. Furthermore, on a stably integrated reporter gene locus, we demonstrate the role of SRSF1 in RNA polymerase II-mediated transcription. Our results suggest that SR proteins mediate the assembly of nuclear speckles and regulate gene expression by influencing both transcriptional and posttranscriptional activities within the cell nucleus.",
author = "Vidisha Tripathi and Song, {David Y.} and Xinying Zong and Shevtsov, {Sergey P.} and Stephen Hearn and Fu, {Xiang Dong} and Miroslav Dundr and Prasanth, {Kannanganattu V.}",
year = "2012",
month = "9",
day = "15",
doi = "10.1091/mbc.E12-03-0206",
language = "English (US)",
volume = "23",
pages = "3694--3706",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "18",

}

TY - JOUR

T1 - SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles

AU - Tripathi, Vidisha

AU - Song, David Y.

AU - Zong, Xinying

AU - Shevtsov, Sergey P.

AU - Hearn, Stephen

AU - Fu, Xiang Dong

AU - Dundr, Miroslav

AU - Prasanth, Kannanganattu V.

PY - 2012/9/15

Y1 - 2012/9/15

N2 - The mammalian cell nucleus is compartmentalized into nonmembranous subnuclear domains that regulate key nuclear functions. Nuclear speckles are subnuclear domains that contain pre-mRNA processing factors and noncoding RNAs. Many of the nuclear speckle constituents work in concert to coordinate multiple steps of gene expression, including transcription, pre-mRNA processing and mRNA transport. The mechanism that regulates the formation and maintenance of nuclear speckles in the interphase nucleus is poorly understood. In the present study, we provide evidence for the involvement of nuclear speckle resident proteins and RNA components in the organization of nuclear speckles. SR-family splicing factors and their binding partner, long noncoding metastasis-associated lung adenocarcinoma transcript 1 RNA, can nucleate the assembly of nuclear speckles in the interphase nucleus. Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and activity of other SR proteins. Furthermore, on a stably integrated reporter gene locus, we demonstrate the role of SRSF1 in RNA polymerase II-mediated transcription. Our results suggest that SR proteins mediate the assembly of nuclear speckles and regulate gene expression by influencing both transcriptional and posttranscriptional activities within the cell nucleus.

AB - The mammalian cell nucleus is compartmentalized into nonmembranous subnuclear domains that regulate key nuclear functions. Nuclear speckles are subnuclear domains that contain pre-mRNA processing factors and noncoding RNAs. Many of the nuclear speckle constituents work in concert to coordinate multiple steps of gene expression, including transcription, pre-mRNA processing and mRNA transport. The mechanism that regulates the formation and maintenance of nuclear speckles in the interphase nucleus is poorly understood. In the present study, we provide evidence for the involvement of nuclear speckle resident proteins and RNA components in the organization of nuclear speckles. SR-family splicing factors and their binding partner, long noncoding metastasis-associated lung adenocarcinoma transcript 1 RNA, can nucleate the assembly of nuclear speckles in the interphase nucleus. Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and activity of other SR proteins. Furthermore, on a stably integrated reporter gene locus, we demonstrate the role of SRSF1 in RNA polymerase II-mediated transcription. Our results suggest that SR proteins mediate the assembly of nuclear speckles and regulate gene expression by influencing both transcriptional and posttranscriptional activities within the cell nucleus.

UR - http://www.scopus.com/inward/record.url?scp=84866427418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84866427418&partnerID=8YFLogxK

U2 - 10.1091/mbc.E12-03-0206

DO - 10.1091/mbc.E12-03-0206

M3 - Article

C2 - 22855529

AN - SCOPUS:84866427418

VL - 23

SP - 3694

EP - 3706

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 18

ER -