SRSF1 regulates the assembly of pre-mRNA processing factors in nuclear speckles

Vidisha Tripathi, David Y. Song, Xinying Zong, Sergey P. Shevtsov, Stephen Hearn, Xiang Dong Fu, Miroslav Dundr, Kannanganattu V. Prasanth

Research output: Contribution to journalArticlepeer-review


The mammalian cell nucleus is compartmentalized into nonmembranous subnuclear domains that regulate key nuclear functions. Nuclear speckles are subnuclear domains that contain pre-mRNA processing factors and noncoding RNAs. Many of the nuclear speckle constituents work in concert to coordinate multiple steps of gene expression, including transcription, pre-mRNA processing and mRNA transport. The mechanism that regulates the formation and maintenance of nuclear speckles in the interphase nucleus is poorly understood. In the present study, we provide evidence for the involvement of nuclear speckle resident proteins and RNA components in the organization of nuclear speckles. SR-family splicing factors and their binding partner, long noncoding metastasis-associated lung adenocarcinoma transcript 1 RNA, can nucleate the assembly of nuclear speckles in the interphase nucleus. Depletion of SRSF1 in human cells compromises the association of splicing factors to nuclear speckles and influences the levels and activity of other SR proteins. Furthermore, on a stably integrated reporter gene locus, we demonstrate the role of SRSF1 in RNA polymerase II-mediated transcription. Our results suggest that SR proteins mediate the assembly of nuclear speckles and regulate gene expression by influencing both transcriptional and posttranscriptional activities within the cell nucleus.

Original languageEnglish (US)
Pages (from-to)3694-3706
Number of pages13
JournalMolecular biology of the cell
Issue number18
StatePublished - Sep 15 2012

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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